ULTRASTRUCTURAL LOCALIZATION OF IMMUNE-COMPLEXES (IGG AND C3) AT ENDPLATE IN EXPERIMENTAL AUTO-IMMUNE MYASTHENIA-GRAVIS
ULTRASTRUCTURAL LOCALIZATION OF IMMUNE-COMPLEXES (IGG AND C3) AT ENDPLATE IN EXPERIMENTAL AUTO-IMMUNE MYASTHENIA-GRAVIS
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DOI：
10.1097/00005072-197803000-00008
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发表时间：
1978-01-01
影响因子：
3.2
通讯作者：
LENNON, VA
中科院分区：
文献类型：
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作者：
SAHASHI, K;ENGEL, AG;LENNON, VA
用纯化的电鳐乙酰胆碱受体（AChR）加佐剂免疫的大鼠在第28天后出现慢性实验性自身免疫性重症肌无力（EAMG）。使用免疫后29-103天EAMG大鼠和对照动物的前肢肌肉进行IG[免疫球蛋白]G和C3 [补体第三组分]的超微结构定位。用兔抗大鼠IgG证明IgG，然后用过氧化物酶标记的葡萄球菌蛋白A处理。用过氧化物酶标记的兔抗大鼠C3或用未标记的兔抗大鼠C3，然后用过氧化物酶标记的蛋白A证明C3。在EAMG大鼠中，IgG和C3定位于连接褶皱的末端扩展，AChR是已知的位置，并在分离，退化的部分，在突触空间的褶皱。背景染色可忽略不计。研究结果提供了明确的证据，涉及EAMG的突触后膜的破坏性自身免疫反应，牵连补体系统在这个反应中，并显示，分离的交界处褶皱的尖端是一种方式，通过这种方式，免疫复合物和AChR从突触后膜消除。慢性EAMG的免疫电镜结果与人类重症肌无力的结果非常相似。
Rats immunized with purified Torpedo acetylcholine receptor (AChR) plus adjuvants developed chronic experimental autoimmune myasthenia gravis (EAMG) after day 28. Forelimb muscles from EAMG rats 29-103 days after immunization and from control animals were used for the ultrastructural localization of Ig[immunoglobulin]G and C3 [3rd component of complement]. IgG was demonstrated with rabbit anti-rat IgG followed by treatment with peroxidase-labeled staphylococcal protein A. C3 was demonstrated with peroxidase-labeled rabbit anti-rat C3, or with unlabeled rabbit anti-rat C3 followed by peroxidase-labeled protein A. In EAMG rats, IgG and C3 were localized on the terminal expansions of the junctional folds, where AChR is known to be located, and on detached, degenerated parts of the folds in the synaptic space. Background staining was negligible. The findings provide unambiguous evidence for a destructive autoimmune reaction involving the postsynaptic membrane in EAMG, implicate the complement system in this reaction and show that detachment of the tips of the junctional folds is one way by which immune complexes and AChR are eliminated from the postsynaptic membrane. The immuno-electron microscopic findings in chronic EAMG closely resemble those described in human myasthenia gravis.