Calcium channels in thrombin-activated human platelet membrane
Calcium channels in thrombin-activated human platelet membrane
复制标题
凝血酶激活的人血小板膜中的钙通道
作者：
A. Zschauer;C. Breemen;F. Bühler;M. Nelson
已知的血小板激活因子、5-羟色胺、血栓素A2、二磷酸腺苷和凝血酶可通过刺激钙通道1、2激活血小板，但通道的性质尚不清楚。我们提出了从凝血酶激活的人血小板中鉴定单一二价阳离子通道的方法。将未经刺激和凝血酶刺激的人血小板膜囊泡掺入平面双层膜中，测量通过单通道的单位电流。只有在凝血酶刺激的制剂中才能显示二价阳离子选择通道。这些通道与电压依赖性钙通道有许多共同的特性--对二价阳离子具有高度的选择性，单通道电导约为10ps(以150 mM Ba2+为单位)，对无机钙通道阻滞剂(如Ni2+)的阻断敏感。在其他方面，这些通道是不同的，因为它们不依赖于电压，也不被1，4-二氢吡啶钙通道拮抗剂阻断。
Platelet-activating factor, 5-hydroxytryptamine, thromboxane A2, adenosine diphosphate and thrombin are known to activate platelets by stimulating calcium entry1,2, but the nature of the entry pathways is unknown3. We present the identification of single divalent cation channels from thrombin-activated human platelets. Membrane vesicles from unstimulated and thrombin-stimulated human platelets were incorporated in planar bilayers and unitary currents through single channels were measured. Divalent cation selective channels could only be demonstrated in thrombin-stimulated preparations. These channels share a number of properties in common with voltage-dependent calcium channels—a high degree of selectivity for divalent cations, a single channel conductance of about 10 pS (in 150 mM Ba2+) and sensitivity to blockade by inorganic calcium channel blockers such as Ni2+. In other respects, these channels are different as they are not voltage-dependent and are not blocked by 1,4-dihydropyridine calcium channel antagonists.
血小板活化中凝血酶诱导的钙运动。
DOI：
10.1016/0167-4889(87)90244-8
发表时间：
1987
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Jy,W;Haynes,DH
通讯作者：
Haynes,DH