Major colour patterns of reef-building corals are due to a family of GFP-like proteins
Major colour patterns of reef-building corals are due to a family of GFP-like proteins
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DOI：
10.1007/pl00006956
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发表时间：
2001-01-01
期刊：
影响因子：
3.5
通讯作者：
Ranganathan, S
中科院分区：
文献类型：
--
作者：
Dove, SG;Hoegh-Guldberg, O;Ranganathan, S
造礁珊瑚以其绚丽的色彩而闻名，但珊瑚色素沉着的生化基础尚不清楚。在这里，我们证明这些颜色是由类 GFP 蛋白家族引起的，它们在紫外线 (UV) 或可见光下发出荧光。来自 10 种珊瑚的色素几乎与 Pocilloporin (Dove et al. 1995) 相同，是具有大约 28 kDa 亚基的二聚体或三聚体。对常见 N 端序列制作的退化引物产生了来自造礁珊瑚 cDNA 的完整序列，其与绿色荧光蛋白 (GFP) 具有 19.6% 的氨基酸同一性。分子模型揭示了“β-can”结构，如 GFP，具有 11 条 β-链和由修饰残基 Gln-61、Tyr-62 和 Gly-63 组成的完全溶剂不可接近的荧光团。波西洛孔素的分子特性表明其具有一系列功能，从将高强度紫外线辐射转化为可通过甲藻甲藻素-叶绿素-蛋白 (PCP) 复合物调节的光合有效辐射 (PAR)，到屏蔽叶绿素 a 和 c 的 Soret 和 Q(x) 带免受散射的高强度光的影响。波西洛孔素的这些特性支持其在强光条件下保护珊瑚共生甲藻光合机制以及在阴凉条件下增强光合光的可用性方面的潜在作用。
Reef-building corals are renowned for their brilliant colours yet the biochemical basis for the pigmentation of corals is unknown. Here, we show that these colours are due to a family of GFP-like proteins that fluoresce under ultraviolet (UV) or visible light. Pigments from ten coral species were almost identical to pocilloporin (Dove et al. 1995) being dimers or trimers with approximately 28-kDa subunits. Degenerative primers made to common N-terminal sequences yielded a complete sequence from reef-building coral cDNA, which had 19.6% amino acid identity with green fluorescent protein (GFP). Molecular modelling revealed a 'beta -can' structure, like GFP, with 11 beta -strands and a completely solvent-inaccessible fluorophore composed of the modified residues Gln-61, Tyr-62 and Gly-63. The molecular properties of pocilloporins indicate a range of functions from the conversion of high-intensity UV radiation into photosynthetically active radiation (PAR) that can be regulated by the dinoflagellate peridinin-chlorophyll-protein (PCP) complex, to the shielding of the Soret and Q(x) bands of chlorophyll a and c from scattered high-intensity light. These properties of pocilloporin support its potential role in protecting the photosynthetic machinery of the symbiotic dinoflagellates of corals under high light conditions and in enhancing the availability of photosynthetic light under shade conditions.