Escherichia coli photoreactivating enzyme: purification and properties.
Escherichia coli photoreactivating enzyme: purification and properties.
复制标题
大肠杆菌光活化酶：纯化和特性。
DOI：
10.1021/bi00559a010
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
Sutherland,BM
中科院分区：
文献类型：
--
作者：
Snapka,RM;Sutherland,BM
切肉刀,1968;Setlow et al., 1969；Sutherland et al., 1970；Hart et al., 1977)。DNA光活化酶（EC4.1）。99.3)在两步反应中修复紫外线照射的DNA。酶与DNA结合（Rupert, 1962），可能是在二聚体上，形成亚稳态复合物。在吸收300-600 nm范围内的光子时，酶催化二聚体的光解（Setlow et al., 1965），从而产生两个单体嘧啶并恢复DNA的生物完整性。
Cleaver, 1968; Setlow et al., 1969; Sutherland et al., 1970; Hart et al., 1977). The DNA photoreactivating enzyme (EC4.1. 99.3) repairs UV'-irradiated DNA in a two-step reaction. The enzyme bindsto the DNA (Rupert, 1962), presumably at the dimer, forming a metastable complex. On absorption of a photon in the range 300-600 nm, the enzyme catalyzes the photolysis of the dimer (Setlow et al., 1965), thus pro-ducing two monomer pyrimidines and restoringbiological integrity to the DNA.