Isolation and purification of uremic middle molecules by multi-step liquid chromatography
Isolation and purification of uremic middle molecules by multi-step liquid chromatography
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DOI：
10.1360/02yb9030
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发表时间：
2002-03
影响因子：
9.6
通讯作者：
Chu Jiegen;Xiaohang Liu;Zhi Yuan;B. He
中科院分区：
文献类型：
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作者：
Chu Jiegen;Xiaohang Liu;Zhi Yuan;B. He
用凝胶渗透层析法、阴离子交换层析法和反相高效液相色谱法对尿毒症患者血清和尿液、正常血清和尿液进行分离比较。用凝胶渗透层析法从尿毒症患者血清、尿液和正常尿液中分离到两个尿毒症中分子组分(A和B)，而正常血清中未分离到。不同来源A组分的阴离子交换层析结果表明，A-3亚组分可在正常人尿液中排泄，但在尿毒症患者肾功能衰竭患者的尿毒症血清中蓄积。脱盐后用MALDI-TOF-MS分析亚组分A-3。结果表明，亚组分A-3由6个化合物组成，其相对分子质量分别为839、873、1007.94、1106、1680和2015年。最后，通过反相高效液相色谱进一步将A-3亚组分分离为6个独立的组分。从而实现了A-3亚组分中六种中分子化合物的分离纯化。
Isolation and comparison of uremic sera and urine and normal sera and urine were performed by gel permeation chromatography, anion exchange chromatography and reversed-phase high performance liquid chromatography. Two uremic middle molecular fractions (A and B) were obtained from uremic sera and urine and normal urine by gel permeation chromatography, but not from normal sera. The anion exchange chromatographic results of fraction A from different origins demonstrate that subfraction A-3 could be excreted in urine by healthy subject, but accumulated in uremic serum for renal failure of patient with uremia. After desalinization subfraction A-3 was analyzed by MALDI-TOF-MS. The results show that subfraction A-3 consists of six compounds with molecular weight 839, 873, 1007.94, 1106, 1680 and 2015 respectively. Finally, by reversed-phase high performance liquid chromatography, subfraction A-3 was further resolved into six independent fractions. Thus, the isolation and purification of six middle molecular compounds in subfraction A-3 came true by our method.