Outer Membrane Vesicles From Probiotic and Commensal Escherichia coli Activate NOD1-Mediated Immune Responses in Intestinal Epithelial Cells.
Outer Membrane Vesicles From Probiotic and Commensal Escherichia coli Activate NOD1-Mediated Immune Responses in Intestinal Epithelial Cells.
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来自益生菌和共生大肠杆菌的外膜囊泡可激活肠上皮细胞中 NOD1 介导的免疫反应。
DOI：
10.3389/fmicb.2018.00498
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发表时间：
2018
影响因子：
5.2
通讯作者：
Baldomà L
中科院分区：
文献类型：
--
作者：
Cañas MA;Fábrega MJ;Giménez R;Badia J;Baldomà L
肠道菌群在维持人肠内稳态和宿主健康方面起着关键作用。细菌带有许多模式识别受体的配体，这些配体是先天性免疫组织化学的关键组成部分。细菌细胞壁中存在的肽聚糖。通过OMV递送到胞质点，这些点是通过内吞作用的。通过微生物群来激活NOD接收器。 -κB和随后的IL-6和IL-8表达。内吞OMV与NOD1共定位，触发NOD1聚集体的形成，并促进NOD1与早期内体的关联。
Gut microbiota plays a critical role in maintaining human intestinal homeostasis and host health. Bacterial extracellular vesicles are key players in bacteria–host communication, as they allow delivery of effector molecules into the host cells. Outer membrane vesicles (OMVs) released by Gram-negative bacteria carry many ligands of pattern recognition receptors that are key components of innate immunity. NOD1 and NOD2 cytosolic receptors specifically recognize peptidoglycans present within the bacterial cell wall. These intracellular immune receptors are essential in host defense against bacterial infections and in the regulation of inflammatory responses. Recent contributions show that NODs are also fundamental to maintain intestinal homeostasis and microbiota balance. Peptidoglycan from non-invasive pathogens is delivered to cytosolic NODs through OMVs, which are internalized via endocytosis. Whether this pathway could be used by microbiota to activate NOD receptors remains unexplored. Here, we report that OMVs isolated from the probiotic Escherichia coli Nissle 1917 and the commensal ECOR12 activate NOD1 signaling pathways in intestinal epithelial cells. NOD1 silencing and RIP2 inhibition significantly abolished OMV-mediated activation of NF-κB and subsequent IL-6 and IL-8 expression. Confocal fluorescence microscopy analysis confirmed that endocytosed OMVs colocalize with NOD1, trigger the formation of NOD1 aggregates, and promote NOD1 association with early endosomes. This study shows for the first time the activation of NOD1-signaling pathways by extracellular vesicles released by gut microbiota.
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影响因子：
3.1
作者：
Bielig, H.;Rompikuntal, P. K.;Kufer, T. A.
通讯作者：
Kufer, T. A.
影响因子：
3.7
作者：
Boonyanugomol W;Chomvarin C;Hahnvajanawong C;Sripa B;Kaparakis-Liaskos M;Ferrero RL
通讯作者：
Ferrero RL
影响因子：
4.8
作者：
Girardin, SE;Travassos, LH;Mengin-Lecreulx, D
通讯作者：
Mengin-Lecreulx, D
影响因子：
5.2
作者：
Alvarez CS;Badia J;Bosch M;Giménez R;Baldomà L
通讯作者：
Baldomà L
影响因子：
30.3
作者：
Irving, Aaron T.;Mimuro, Hitomi;Kaparakis-Liaskos, Maria
通讯作者：
Kaparakis-Liaskos, Maria