Carbon release from purified chemoautotrophic bacterial symbionts of the hydrothermal vent tubeworm Riftia pachyptila
Carbon release from purified chemoautotrophic bacterial symbionts of the hydrothermal vent tubeworm Riftia pachyptila
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DOI：
10.1086/515931
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发表时间：
1998-05-01
期刊：
影响因子：
--
通讯作者：
Jarchow, J
中科院分区：
文献类型：
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作者：
Felbeck, H;Jarchow, J
无肠热液管虫 Riftia pachyptila Tones 主要依靠其化能自养细菌共生体以固定和掺入二氧化碳产生的分泌有机化合物的形式提供营养。在这项研究中，共生体被纯化，测试活力，并在标记的二氧化碳存在下孵育。我们证明，纯化的共生体可以用作细菌培养实验的可行替代方案。几种有机酸、糖和氨基酸被标记，但它们占总标记的比例在所用的孵育时间内基本保持不变。然而，越来越多的琥珀酸盐和较小程度的谷氨酸被分泌到培养介质中，表明这些可能是从共生体转移到宿主的主要含碳化合物。谷氨酸还可以解释氮从共生体到宿主的运输。
The gutless hydrothermal tubeworm Riftia pachyptila Tones relies mainly on its chemoautotrophic bacterial symbionts to supply nutrients in the form of secreted organic compounds resulting from fixation and incorporation of CO2. In this study, symbionts were purified, tested for viability, and incubated in the presence of labeled CO2. We demonstrated that purified symbionts can be used as a viable alternative to experiments with bacterial cultures. Several organic acids, sugars, and amino acids were labeled, but their fraction of the total label stayed generally constant during the incubation times used. However, increasing fractions of succinate and, to a lesser degree, glutamate were excreted into the incubation medium, indicating that these are probably the main carbon-containing compounds transferred from the symbionts to the host. Glutamate could also account for the transport of nitrogen from the symbionts to the host.