Development of an agar-methyl cellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder.
Development of an agar-methyl cellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder.
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开发人膀胱移行细胞癌细胞的琼脂甲基纤维素克隆形成测定法。
作者：
R. Buick';T. Stanisic;S. Fry;S. Salmon;J. Trent;P. Krasovich
我们报道了一种用于膀胱移行细胞癌祖细胞克隆形成检测的方法。集落生长已被证明来自手术活检和膀胱barbotages获得的细胞。电子显微镜和核型证据支持这样的论点，即这些祖细胞代表了维持体内肿瘤的群体的一部分。11例手术活检样本中有9例出现菌落生长，6例膀胱穿刺样本中有6例出现菌落生长。接种效率高达0.7%，在某些情况下集落大小大于1000个细胞。该测定法似乎对分析人移行细胞癌的生物学具有潜在的用途。
We report the development of a clonogenic assay for progenitor cells in transitional cell carcinoma of the bladder. Colony growth has been demonstrated from cells obtained both from surgical biopsies and from bladder barbotages. Electron microscopic and karyotypic evidence supports the contention that these progenitors represent a part of the population maintaining the tumor in vivo. Colony growth occurred in 9 of 11 surgical biopsy samples and in 6 of 6 bladder barbotage samples. Plating efficiency ranged up to 0.7%, and colony size was in some instances greater than 1000 cells. The assay appears potentially useful for analysis of the biology of human transitional cell carcinoma.