The Impacts of ACE Activity according to ACE I/D Polymorphisms on Muscular Functions of People Aged 65.
The Impacts of ACE Activity according to ACE I/D Polymorphisms on Muscular Functions of People Aged 65.
复制标题
DOI：
10.5535/arm.2012.36.4.433
复制
发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Woo JH
中科院分区：
文献类型：
--
作者：
Kang HJ;Kim CH;Park DS;Choi SY;Lee DH;Nam HS;Hur JG;Woo JH
研究65岁韩国人血管紧张素转换酶（ACE）多态性与肌肉疲劳性之间的关系。研究对象为49名65岁的韩国人。聚合酶链反应检测ACE插入/缺失（I/D）多态性，分光光度法测定血清ACE活性。测定体重指数（BMI）、体脂量（BFM）和瘦体重（LBM）。为了评估肌肉疲劳性，动态肌电图被用来测量踝跖屈肌的最大随意等长收缩（MVIC）。患者坐位，髋关节屈曲90°，膝关节完全伸展，踝关节0°。然后进行连续次最大VIC（40%MVIC），并测量最初2分钟内的收缩持续时间和EMG频率变化。自我报告的体力活动问卷被用来评估ACE活动水平对肌肉疲劳的影响。49名志愿者中，15名为II型，22名为ID型，12名为DD型。DD基因型患者血清ACE活性显著高于II基因型患者（p<0.05）。此外，II和ID基因型受试者的次最大等长收缩持续时间长于DD基因型受试者（p<0.05）。动态肌电图显示II基因型受试者的平均频率变化显著低于DD基因型受试者（p<0.05）。然而，LBM、BFM和BMI与ACE基因型无关。ACE Ⅱ基因型个体的抗疲劳能力显著高于DD基因型个体。然而，身体成分和BMI与ACE I/D多态性无关。
To investigate associations between angiotensin-converting enzyme (ACE) polymorphisms and muscle fatigability in 65-year-old Koreans. The study participants were 49 Koreans aged 65 years. ACE insertion/deletion (I/D) polymorphisms were determined by polymerase chain reaction and serum ACE activity, by spectrophotometry. Body mass index (BMI), body fat mass (BFM), and lean body mass (LBM) were determined. To evaluate muscle fatigability, dynamic Electromyography was used to measure maximum voluntary isometric contractions (MVICs) of ankle plantar flexor muscles. Patients were seated with their hips flexed at 90°, knees fully extended, and ankles at 0°. Continuous submaximal VICs (40% MVIC) were then performed, and contraction duration and EMG frequency changes during the initial 2 min were measured. A self-reported physical activity questionnaire was used to evaluate effects of ACE activity levels on muscle fatigability. Among the 49 volunteers, 15 showed II genotype; 22, ID genotype; and 12, DD genotype. Serum ACE activity levels were significantly higher in DD genotype subjects than in II genotype subjects (p<0.05). Furthermore, the duration of submaximal isometric contractions was longer in II and ID genotype subjects than in DD genotype subjects (p<0.05). Dynamic EMG showed significantly lower mean frequency changes in II genotype subjects than in DD genotype subjects (p<0.05). However, LBM, BFM, and BMI were independent of ACE genotypes. ACE II genotype subjects showed significantly higher resistant to muscle fatigue than that by DD genotype subjects. However, body composition and BMI showed no correlations with ACE I/D polymorphisms.