PLASMIDS FROM STAPHYLOCOCCUS-AUREUS REPLICATE IN YEAST SACCHAROMYCES-CEREVISIAE
PLASMIDS FROM STAPHYLOCOCCUS-AUREUS REPLICATE IN YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1038/298488a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
EHRLICH, SD
中科院分区：
文献类型：
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作者：
GOURSOT, R;GOZE, A;EHRLICH, SD
已知一些质粒，如RP 4，可以在许多革兰氏阴性菌中复制1。某些小的金黄色葡萄球菌质粒具有更广泛的宿主范围，不仅能够在遗传学上遥远的革兰氏阳性菌（如枯草芽孢杆菌2或肺炎链球菌3）中复制，而且能够在革兰氏阴性菌（大肠杆菌4）中复制。在这里，我们已经研究了这些质粒是否也可以复制在一个较低的真核生物，酵母酿酒酵母。为此，我们构建了aS之间的混合体。aureus质粒pC 194和一个E.大肠杆菌质粒YIp 5携带aura-3基因，该基因在酵母中易于选择，但在该宿主中不能复制。我们发现，杂交体以高效率转化酵母（YIP 5和其他三个之间的杂交体也是如此）。金黄色质粒）;在酵母中保持在染色体外;并且在酵母中停留期间不被修饰。我们从这个证据得出结论，S。金黄色质粒可以在酵母中复制，这就提出了原核生物和真核生物使用的复制信号是否相似，以及这些生物在系统发育树上的位置有多高的问题。金色质粒可能是。
It is known that some plasmids, such as RP4, can replicate in many Gram-negative bacteria1. Certain smallStaphylococcus aureusplasmids have an even broader host range, being able to replicate in not only phylogenetically distant Gram-positive bacteria such asBacillus subtilis2orStreptococcus pneumoniae3, but also in the Gram-negative bacteriumEscherichia coli4. Here we have examined whether these plasmids can also replicate in a lower eukaryote, the yeastSaccharomyces cerevisiae. For this purpose we constructed hybrids between aS. aureusplasmid pC194 and anE. coliplasmid YIp5, which carries aura-3gene easy to select for in yeast but cannot replicate in this host. We found that the hybrids transformed yeast with high efficiency (as did hybrids between YIp5 and three otherS. aureusplasmids); were maintained extrachromosomally in yeast; and were not modified during residence in yeast. We conclude from this evidence thatS. aureusplasmids can replicate in yeast, which raises the questions of whether the replication signals used by prokaryotes and eukaryotes are similar, and how far up the phylogenetic tree the organisms still able to be hosts toS. aureusplasmids may be.