Enhanced renal immunocytochemical expression of ANG-(1-7) and ACE2 during pregnancy
Enhanced renal immunocytochemical expression of ANG-(1-7) and ACE2 during pregnancy
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DOI：
10.1161/01.hyp.0000085220.53285.11
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发表时间：
2003-10-01
期刊：
影响因子：
8.3
通讯作者：
Ferrario, CM
中科院分区：
文献类型：
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作者：
Brosnihan, KB;Neves, LAA;Ferrario, CM
以前，我们证明，肾脏浓度和尿排泄的血管紧张素-（1-7）在正常妊娠大鼠增加。由于这一发现可能反映了局部肾脏血管紧张素-（1-7）的产生，我们确定了免疫细胞化学分布的血管紧张素-（1-7）和它的新描述的加工酶，ACE 2，在处女和19天怀孕的Sprague-Dawley大鼠的肾脏。在妊娠第19天处死Sprague-Dawley大鼠，并通过使用血管紧张素-（1-7）的多克隆抗体或ACE 2的单克隆抗体制备组织用于免疫细胞化学。血管紧张素-（1-7）免疫染色主要定位于穿过内皮质和外髓质的肾小管。ACE 2免疫染色定位于整个皮质和外髓，并在处女大鼠和妊娠大鼠的肾小管中可见。血管紧张素-（1-7）和ACE-2免疫细胞化学染色的定量结果显示，妊娠动物的血管紧张素-（1- 7）和ACE-2免疫细胞化学染色强度分别增加56%和117%（P
Previously we demonstrated that kidney concentration and urinary excretion of angiotensin-(1-7) are increased during normal pregnancy in rats. Since this finding may reflect local kidney production of angiotensin-(1-7), we determined the immunocytochemical distribution of angiotensin-(1-7) and its newly described processing enzyme, ACE2, in kidneys of virgin and 19-day-pregnant Sprague-Dawley rats. Sprague-Dawley rats were killed at the 19th day of pregnancy, and tissues were prepared for immunocytochemical by using a polyclonal antibody to angiotensin-(1-7) or a monoclonal antibody to ACE2. Angiotensin-(1-7) immunostaining was predominantly localized to the renal tubules traversing both the inner cortex and outer medulla. ACE2 immunostaining was localized throughout the cortex and outer medulla and was visualized in the renal tubules of both virgin and pregnant rats. The quantification of angiotensin-(1-7) and ACE2 immunocytochemical staining showed that in pregnant animals, the intensity of the staining increased by 56% and 117%, respectively (P