PTX3: an inflammatory protein modulating ultrastructure and bioenergetics of human endothelial cells
PTX3: an inflammatory protein modulating ultrastructure and bioenergetics of human endothelial cells
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DOI：
10.1186/s12979-019-0144-0
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发表时间：
2019-02-02
影响因子：
7.9
通讯作者：
Vecchione, Carmine
中科院分区：
文献类型：
--
作者：
Carrizzo, Albino;Procaccini, Claudio;Vecchione, Carmine
PTX3是一种由多种细胞产生的急性期炎症蛋白，长期以来一直被认为是年龄相关性心脑血管疾病的可能生物标志物。尽管到目前为止，在血管和免疫系统中已经确定了几种作用机制，但PTX3在形态和代谢水平上对分离的内皮细胞的直接影响尚不清楚。发现PTX3诱导分离的人内皮细胞胞浆空泡化和线粒体基质稀释。此外，代谢分析表明，PTX3增加了支持线粒体功能的呼吸能力，并部分维持了糖酵解途径。结论PTX3本身对分离的内皮细胞的超微结构和生物能量参数具有直接作用。这一发现可能与我们之前演示的PTX3对内皮层的有害影响有关。需要更多的研究来明确证明这些超微结构和生物能量变化与内皮功能障碍之间的任何直接相关性，特别是在与年龄相关的脑血管疾病方面。
BackgroundPentraxin 3 (PTX3), an acute-phase inflammation protein produced by several cell types, has long been described as a possible biomarker for age-related cardiovascular and cerebrovascular diseases. Although several mechanisms of action have been identified to date in the vascular and immune systems, the direct effects of PTX3 on isolated endothelial cells at morphological and metabolic levels remain unknown.FindingsPTX3 induced cytoplasmic vacuolization and dilution of mitochondrial matrix in isolated, human endothelial cells. Moreover, metabolic assays revealed that PTX3 increases respiratory capacity in support of mitochondrial function, and partially sustains the glycolytic pathway.ConclusionsPTX3 has, per se, a direct action on ultrastructural and bioenergetic parameters of isolated endothelial cells. This finding can be associated with our previous demonstration of a deleterious effect of PTX3 on the endothelial layer. More studies are needed to clearly demonstrate any direct correlation between these ultrastructural and bioenergetic changes with endothelial dysfunction, especially with regard to age-related cerebro- and cardio-vascular diseases.