Detection of freshwater mussels (Sinanodonta spp.) in artificial ponds through environmental DNA: a comparison with traditional hand collection methods.
Detection of freshwater mussels (Sinanodonta spp.) in artificial ponds through environmental DNA: a comparison with traditional hand collection methods.
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通过环境 DNA 检测人工池塘中的淡水贻贝（Sinanodonta spp.）：与传统手工采集方法的比较。
DOI：
10.1007/s10201-019-00584-0
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发表时间：
2020
期刊：
影响因子：
1.6
通讯作者：
and Katano I.
中科院分区：
文献类型：
--
作者：
Togaki D;Doi H;and Katano I.
大多数淡水贻贝的数量在世界范围内都在下降；其中包括栖息在日本人工池塘中的中华贻贝。环境DNA(EDNA)的检测将是一种快速、有效的监测华南齿类种群分布的方法。因此，本研究的目的是比较基于实时聚合酶链式反应的EDNA检测结果和日本24个池塘人工采集的结果。手工采集的体表中华绒毛虫的数量与EDNA的检出率呈正相关。这些发现表明，EDNA检测是一种有价值的替代方法，可以用来评估华支睾吸虫的分布，并且该方法具有与手工采集相似的检测灵敏度。EDNA方法可用于在人工采集困难的大范围和令人望而却步的深度监测淡水贻贝。
Populations of most freshwater mussels (Unionidae) are declining worldwide; these includesSinanodontaspp., which inhabit artificial ponds in Japan. The detection of environmental DNA (eDNA) would be a rapid and efficient method for monitoring the distribution ofSinanodontapopulations. Accordingly, the aim of the present study was to compare the results of real-time PCR-based eDNA detection to those of hand collection in 24 ponds in Japan.SinanodontaeDNA was detected in most of the study ponds whereSinanodontaspp. were found by hand collection, and there was a positive correlation between the eDNA detection rate and the number ofSinanodontaspecimens collected by hand. These findings demonstrate that eDNA detection is a valuable alternative method of evaluating the distribution ofSinanodontaspp., and that this method has a similar detection sensitivity to that of hand collection. The eDNA method can be used to monitor freshwater mussels at broad scales and prohibitive depths where hand collection is difficult.