Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth.
Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth.
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DOI：
10.1038/srep23575
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发表时间：
2016-03-31
影响因子：
4.6
通讯作者：
Bhattacharyya MS
中科院分区：
文献类型：
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作者：
Haque F;Alfatah M;Ganesan K;Bhattacharyya MS
白色念珠菌引起浅表和危及生命的全身感染。这些是很难治疗的，往往是由于耐药性，特别是因为C。白色念珠菌生物膜固有地对大多数抗真菌剂具有抗性。槐糖脂（SL）是一种糖脂生物表面活性剂，具有抗菌和抗癌作用。在这项研究中，我们研究了SL对C。白色念珠菌生物膜形成和预形成的生物膜。SL对C.白色念珠菌生物膜形成以及降低预形成的生物膜的生存力。此外，发现SL当与阿替霉素B（AmB）或氟康唑（FLZ）一起沿着使用时，协同作用对抗生物膜形成和预形成的生物膜。SL对C.通过扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）进一步观察白色念珠菌生物膜形成，其揭示了菌丝的缺乏、典型的生物膜结构和生物膜细胞形态的改变。同时发现SL下调了菌丝特异性基因HWP 1、ALS 1、ALS 3、ECE 1和SAP 4的表达，这可能解释了SL对菌丝和生物膜形成的抑制作用。
Candida albicans causes superficial and life-threatening systemic infections. These are difficult to treat often due to drug resistance, particularly because C. albicans biofilms are inherently resistant to most antifungals. Sophorolipid (SL), a glycolipid biosurfactant, has been shown to have antimicrobial and anticancer properties. In this study, we investigated the effect of SL on C. albicans biofilm formation and preformed biofilms. SL was found to inhibit C. albicans biofilm formation as well as reduce the viability of preformed biofilms. Moreover, SL, when used along with amphotericin B (AmB) or fluconazole (FLZ), was found to act synergistically against biofilm formation and preformed biofilms. Effect of SL on C. albicans biofilm formation was further visualized by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), which revealed absence of hyphae, typical biofilm architecture and alteration in the morphology of biofilm cells. We also found that SL downregulates the expression of hypha specific genes HWP1, ALS1, ALS3, ECE1 and SAP4, which possibly explains the inhibitory effect of SL on hyphae and biofilm formation.