Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting Microbes
Agricultural Utilization of Unused Resources: Liquid Food Waste Material as a New Source of Plant Growth-Promoting Microbes
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DOI：
10.3390/agronomy10070954
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发表时间：
2020-07
期刊：
影响因子：
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通讯作者：
Waleed Asghar;Shiho Kondo;Riho Iguchi;A. Mahmood;R. Kataoka
中科院分区：
文献类型：
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作者：
Waleed Asghar;Shiho Kondo;Riho Iguchi;A. Mahmood;R. Kataoka
有机改良剂通过提高土壤肥力和土壤微生物来改善土壤质量。本研究通过分析液态食物垃圾（LFM）中的微生物群落组成，并从液态食物垃圾中分离植物生长促进菌（PGPB），评价了液态食物垃圾作为微生物资源的有效性。2018年5 - 9月每月采集LFM的高通量测序结果显示，LFM中检测到细菌操作分类单位（OTUs）为bb101000个。结果表明，厚壁菌门（Firmicutes）数量最多，检测频率最高，其次是变形菌门（Proteobacteria）和放线菌门（Actinobacteria）。从LFM中分离出的可培养菌株几乎都属于芽孢杆菌属。从分离菌株中筛选出4株具有吲哚乙酸产量和1-氨基环丙烷-1-羧酸脱氨酶活性的PGPB菌株。施用ppgpb对生菜生长有促进作用，与未施用化肥相比，施用LFM对油菜根系生物量有显著影响。田间试验表明，与施用化肥相比，LFM对油菜、莴苣和茄子的生长有轻微的影响。LFM是分离PGPB的有益微生物资源，将其用作肥料可以减少可持续农业中化肥的使用。
Organic amendment is important for promoting soil quality through increasing soil fertility and soil microbes. This study evaluated the effectiveness of using liquid food waste material (LFM) as a microbial resource, by analyzing the microbial community composition in LFM, and by isolating plant growth-promoting bacteria (PGPB) from the material. High-throughput sequencing of LFM, collected every month from May to September 2018, resulted in the detection of >1000 bacterial operational taxonomic units (OTUs) in the LFM. The results showed that Firmicutes was abundant and most frequently detected, followed by Proteobacteria and Actinobacteria. Of the culturable strains isolated from LFM, almost all belonged to the genus Bacillus. Four strains of PGPB were selected from the isolated strains, with traits such as indole acetic acid production and 1-aminocyclopropane-1-carboxylic acid deaminase activity. Lettuce growth was improved via LFM amendment with PGPB, and Brassica rapa showed significant differences in root biomass when LFM amendment was compared with the use chemical fertilizer. Field experiments using LFM showed slight differences in growth for Brassica rapa, lettuce and eggplant, when compared with the use of chemical fertilizer. LFM is a useful microbial resource for the isolation of PGPB, and its use as fertilizer could result in reduced chemical fertilizer usage in sustainable agriculture.