Uptake of dioxin-like compounds from sewage sludge into various plant species - assessment of levels using a sensitive bioassay
Uptake of dioxin-like compounds from sewage sludge into various plant species - assessment of levels using a sensitive bioassay
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DOI：
10.1016/s0045-6535(99)00368-9
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发表时间：
2000-05-01
期刊：
影响因子：
8.8
通讯作者：
Hjelm, K
中科院分区：
文献类型：
--
作者：
Engwall, M;Hjelm, K
一种用于检测二恶英类化合物的生物测定法被用来估计胡萝卜、油菜籽、西葫芦和黄瓜对二恶英类化合物的吸收，这些胡萝卜、油菜籽、西葫芦和黄瓜生长在用瑞典污水处理厂（STP）的污水污泥改良的土壤中，这种灵敏的生物测定是基于培养的鸡胚肝脏中7-乙氧基试卤灵O-脱乙基酶（EROD）的诱导作用，反映了所有二恶英的综合生物效应，比如样品中的化合物，包括那些很少被分析的化合物。生物测定在所有胡萝卜、西葫芦和黄瓜样品中检测到低浓度的二恶英类化合物，但在油菜种子中没有检测到任何二恶英类化合物。在胡萝卜的浓度增加了7倍，当生长在土壤中修改与高应用程序的一些污泥样品，而其他人没有增加的浓度相比，控制。更现实的污泥应用只是稍微增加了浓度。污泥施肥的胡萝卜含有最高浓度的被调查植物（高达14皮克生物测定衍生的四氯二苯并对二恶英当量（生物TEQs）/克干重）。在胡萝卜中，二恶英类化合物的吸收差异取决于污泥来源，这可能是由于更容易生物积累的二恶英类化合物在一些污泥样品，或其他成分，促进吸收到胡萝卜。在黄瓜中，增加了两倍多（从0.2至0.5皮克生物毒性当量/克干重）。的污泥修正土壤中生长的标本中观察到的相比，控制，这表明一个小的吸收从根到芽。在西葫芦果实中没有看到污泥依赖的吸收增加。生物毒性当量水平普遍较低的消费地上植物部分的调查物种。然而，重复施用污泥是否会导致二恶英类化合物在土壤中积累，从而增加植物吸收的风险，这一问题仍有待调查。(C)2000爱思唯尔科技有限公司版权所有。
A bioassay for the detection of dioxin-like compounds was used to estimate uptake of dioxin-like compounds in carrots, oil seed rape seeds, zucchinis and cucumbers grown in soil amended with sewage sludge from Swedish sewage treatment plants (STP), This sensitive bioassay is based on 7-ethoxyresorufin O-deethylase (EROD)-induction in cultured chicken embryo livers and reflects the combined biological effect of all dioxin-like compounds in a sample, including ones that seldom are analyzed. The bioassay detected low concentrations of dioxin-like compounds in all carrot, zucchini and cucumber samples, but did not detect any dioxin-like compounds in the rape seeds. In carrots the concentrations were increased up to seven times when grown in soil amended with high applications of some of the sludge samples, while others did not increase the concentrations compared to control. More realistic sludge applications only increased the concentrations slightly. The sludge-fertilized carrots contained the highest concentrations of the investigated plants (up to 14 pg bioassay-derived TCDD equivalents (bio-TEQs)/g d.w.). In the carrots, differences in uptake of dioxin-like compounds depended on the sludge origin, which may be due to more easily bioaccumulated dioxin-like compounds in some sludge samples, or other components that facilitated uptake into the carrots. In the cucumbers, a more than two-fold increase (from 0.2 to 0.5 pg bio-TEQs/g d.w.) was observed in specimens grown in sludge-amended soil when compared to controls, suggesting a small uptake from the roots to the shoots. No sludge-dependent increase in uptake was seen in the zucchini fruits. The bio-TEQ levels were generally low in the consumable above ground plant parts of the investigated species. However, the question if repeated sludge application results in a soil accumulation of dioxin-like compounds, thereby increasing the risk of plant uptake, remains to be investigated. (C) 2000 Elsevier Science Ltd. All rights reserved.