Lactic acid production directly from starch in a starch-controlled fed-batch operation using Lactobacillus amylophilus

Lactic acid production directly from starch in a starch-controlled fed-batch operation using Lactobacillus amylophilus
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使用解淀粉乳杆菌在淀粉控制的分批补料操作中直接从淀粉生产乳酸

DOI:
10.1007/s00449-010-0426-6
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发表时间:
2010
影响因子:
3.8
通讯作者:
Jia
Jia
中科院分区:
工程技术3区
文献类型:
--
作者:
Hong;Jia

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根据批次结果,我们构建了一个简化的同步糖化发酵 (SSF) 模型,用于模拟使用解淀粉乳杆菌直接从未水解的马铃薯淀粉生产乳酸。不同初始淀粉浓度(20、40 和 60 g/l)下的批量操作结果表明,较高的初始淀粉浓度会导致生产率稍低,但会大幅降低产量。其中,初始淀粉含量为 20 g/l 的批次具有最大生产率和最大收率,分别为 0.31 g/(l h) 和 98% (g/g)。为了提高生产率和最终乳酸浓度,采用 20 g/l 初始淀粉进行淀粉控制的分批补料操作。结果表明,通过调节淀粉加料速率将淀粉控制在 8 ± 1 g/l 的分批补料操作可实现最大生产率 0.75 g/(l h) 和 69% 的产率。
Based on the batch results, we constructed a simplified simultaneous saccharification and fermentation (SSF) model for the simulation of lactic acid production directly from unhydrolyzed potato starch usingLactobacillus amylophilus. The results of batch operation at different initial starch concentrations (20, 40 and 60 g/l) indicated that a higher initial starch concentration would lead to a slightly lower productivity, but would largely decrease the yield. Among that, the batch with 20 g/l of initial starch had the maximum productivity and the maximum yield, which would be 0.31 g/(l h) and 98% (g/g), respectively. In view of increasing the productivity and the final lactic acid concentration, a starch-controlled fed-batch operation with 20 g/l of initial starch was performed. It showed the fed-batch operation with starch controlled at 8 ± 1 g/l by adjusting the starch-feeding rate led to the maximum productivity of 0.75 g/(l h) and the yield of 69%.