Low revascularization of experimentally transplanted human pancreatic islets
Low revascularization of experimentally transplanted human pancreatic islets
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DOI：
10.1210/jc.2002-020728
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发表时间：
2002-12-01
影响因子：
5.8
通讯作者：
Mattsson, G
中科院分区：
文献类型：
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作者：
Carlsson, PO;Palm, F;Mattsson, G
胰岛在移植后即刻是无血管的。尽管胰岛迅速血管化，但血管化是否能使移植的胰岛组织充分氧合尚不确定。在移植到裸鼠体内一个月后，我们测量了小鼠或人胰岛的pO（2）、血流量和血管密度。用Clark微电极测定组织pO（2）。用激光多普勒流式细胞术测量血液灌注，而用凝集素Bandeiraea simplicifolia（BS-1）染色的组织学标本测定血管密度。移植的小鼠和人胰岛的pO（2）均为内源性小鼠胰岛的15-20%。此外，与内源性小鼠和人胰岛相比，移植胰岛的血管密度降低。移植物血液灌注约为肾皮质血流量的50%。供者年龄与胰岛移植物血流灌注呈负相关。供体年龄和移植血管总密度之间也存在类似的相关性。总之，与内源性胰岛相比，移植的人胰岛具有显著降低的血管密度和pO（2）。这对临床胰岛移植有潜在的意义，因为不良的血管植入可能会显着增加获得胰岛素依赖性所需的胰岛数量。
Pancreatic islets are avascular immediately after transplantation. Although the islets are rapidly revascularized, it is uncertain whether the revascularization produces an adequate oxygenation of the transplanted islet tissue. We measured pO(2), blood flow and vascular density in mouse or human islets I month after transplantation to nude mice. Tissue pO(2) was measured with Clark microelectrodes. Blood perfusion was measured with laser-Doppler flow cytometry, whereas vascular density was determined in histological specimens stained for the lectin Bandeiraea simplicifolia (BS-1). Both the transplanted mouse and human islets had a pO(2) 15-20% of that in endogenous mouse islets. Moreover, the vascular density of the transplanted islets was decreased compared with that of endogenous mouse and human islets. Graft blood perfusion was approximately 50% of renal cortex blood flow. A negative correlation was found between donor age and blood perfusion of the human islet grafts. A similar correlation was seen between donor age and the total vascular density of these grafts. In conclusion, transplanted human islets had a markedly decreased vascular density and pO(2) compared with endogenous islets. This has potential implications for clinical islet transplantations, because poor vascular engraftment may significantly increase the number of islets needed to obtain insulin independence.