Genomics and homeostasis
Genomics and homeostasis
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DOI：
10.1152/ajpregu.00567.2002
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发表时间：
2003-03-01
影响因子：
2.8
通讯作者：
Cowley, AW
中科院分区：
文献类型：
--
作者：
Cowley, AW
今年的 Cannon 讲座阐释了复杂生物体 DNA 序列的知识如何开始塑造 21 世纪的生理学格局。对于生理学家来说，将大量基因与正常和病理功能联系起来现在面临着巨大的挑战和机遇。去年完成了第一个针对心血管和肾功能的广泛基因组系统生物学图谱，以及一个新的假设生成工具（“生理分析”），使我们能够假设负责调节途径的特定基因之间的关系。染色体替代技术（同体大鼠和同系大鼠）开始确认连锁分析研究的统计结果，缩小定位克隆的遗传兴趣区域，并为生理学研究提供遗传上明确的对照菌株。通过微阵列鉴定的基因表达模式以及将表达基因映射到染色体位点增加了对系统生理学的理解。将大约 36,000 个基因与哺乳动物系统的复杂功能连接起来这一以前难以想象的目标确实正在顺利进行。
The Cannon lecture this year illustrates how knowledge of DNA sequences of complex living organisms is beginning to shape the landscape of physiology in the 21st century. Enormous challenges and opportunities now exist for physiologists to relate the galaxy of genes to normal and pathological functions. The first extensive genomic systems biology map for cardiovascular and renal function was completed last year as well as a new hypothesis-generating tool ("physiological profiling") that enables us to hypothesize relationships between specific genes responsible for the regulation of regulatory pathways. Techniques of chromosomal substitution (consomic and congenic rats) are beginning to confirm statistical results from linkage analysis studies, narrow the regions of genetic interest for positional cloning, and provide genetically well-defined control strains for physiological studies. Patterns of gene expression identified by microarray and mapping of expressed genes to chromosomal sites are adding to the understanding of systems physiology. The previously unimaginable goal of connecting similar to36,000 genes to the complex functions of mammalian systems is indeed well underway.