Development of polymorphic microsatellite markers in the medicinal plant Gardenia jasminoides (Rubiaceae)
Development of polymorphic microsatellite markers in the medicinal plant Gardenia jasminoides (Rubiaceae)
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DOI：
10.1016/j.bse.2014.11.009
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发表时间：
2015-02-01
影响因子：
1.6
通讯作者：
Yang, Chun-xia
中科院分区：
文献类型：
--
作者：
Deng, Shao-yong;Wang, Xian-rong;Yang, Chun-xia
利用基于Illumina的EST序列在药用植物栀子（Gardenia jasminoides）的基因组中鉴定了总共12，960个简单序列重复（SSR）基序。在SSR中，单核苷酸的丰度最高（56.7%），其次是二核苷酸（19%）和三核苷酸（16.4%）。AG/TC（60.2%）、TTC/AAG（25.8%）和TTTC/GAAA（35.9%）重复基序分别是二、三和四核苷酸基序中最丰富的。随后，25个等位基因，多态性引物对进行了鉴定和测试的153个个体从5个自然群体的G。茉莉花每个多态位点的等位基因数（A）范围为2 - 8。观察到的和预期的杂合性变化范围分别为0.095至0.857和0.182至0.832。各位点的PIC值在0.171 ~ 0.792之间。这些新的多态性EST-SSR标记将有助于进一步的遗传研究，这种经济上重要的植物。(C)2014爱思唯尔有限公司版权所有。
A total of 12,960 simple sequence repeats (SSR) motifs were identified in the genome of the medicinal plant Gardenia jasminoides using Illumina-based EST sequences. Among the SSRs, mono-nucleotides were the most abundant (56.7%), followed by di- (19%) and trinucleotides (16.4%). AG/TC (60.2%), TTC/AAG (25.8%), and TTTC/GAAA (35.9%) repeat motifs were the most abundant of the di-, tri- and tetra-nucleotide motifs, respectively. Subsequently, twenty-five allelic, polymorphic primer pairs were identified and tested in 153 individuals from five natural populations of G. jasminoides. The number of alleles per polymorphic locus (A) ranged from two to eight. Observed and expected heterozygosity varied from 0.095 to 0.857 and 0.182 to 0.832, respectively. The PIC values for each locus ranged from 0.171 to 0.792. These new polymorphic EST-SSR markers will be useful for further genetic studies on this economically important plant. (C) 2014 Elsevier Ltd. All rights reserved.