Thermal stress and energy metabolism in two circumtropical decapod crustaceans: Responses to acute temperature events.
Thermal stress and energy metabolism in two circumtropical decapod crustaceans: Responses to acute temperature events.
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DOI：
10.1016/j.marenvres.2018.08.015
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发表时间：
2018-10
影响因子：
3.3
通讯作者：
C. Madeira;M. Leal;M. Diniz;H. Cabral;C. Vinagre
中科院分区：
文献类型：
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作者：
C. Madeira;M. Leal;M. Diniz;H. Cabral;C. Vinagre
与全球变暖有关的极端事件，如海洋热浪，可能对不同物种产生截然不同的适应性后果，从而改变海洋群落的结构和组成。在这里，我们研究了实验室模拟热浪对两种印度-太平洋甲壳类动物生理和性能的影响：虾Rhynchocinetes durbanensis和寄居蟹Calcinus laevimanus。我们将甲壳类动物连续两周暴露于控制温度或+5 °C温度（25 °C vs 30 °C），每周分析肌肉和内脏质量中的保护蛋白，抗氧化活性和脂质过氧化物。在实验结束时还分析了肌肉组织的富尔顿K、总蛋白、%C和C：N摩尔比。结果表明：（1）对虾的肌肉和寄居蟹的内脏质量是最敏感的组织;（2）两种生物标志物的反应主要发生在暴露7 d后;（3）温度胁迫导致生物标志物水平升高;（4）保护性分子伴侣和抗氧化剂超氧化物歧化酶和谷胱甘肽-S-转移酶的生物标志物倍数变化最大; 4）综合生物标志物指数表明，遭受热浪的个体的健康状况较差; 5）仅在R中检测到生物体水平的性能变化。（6）两个物种的死亡率在热浪中保持不变。最后，我们得出的结论是，这些物种能够根据快速的环境变化进行生理调整，这最终赋予它们足够的耐热性来抵御这种模拟热浪，而不会对适应性产生重大影响。
Extreme events associated with global warming, such as ocean heat waves, can have contrasting fitness consequences for different species, thereby modifying the structure and composition of marine communities. Here, we examined the effects of a laboratory simulated heat wave on the physiology and performance of two Indo-Pacific crustacean species: the shrimpRhynchocinetes durbanensisand the hermit crabCalcinus laevimanus. We exposed the crustaceans to a control temperature or to a +5 °C temperature (25 °C vs 30 °C) for two consecutive weeks, and weekly analyzed protective proteins, antioxidant activity, and lipid peroxides in muscle and visceral mass. Fulton's K, total protein, %C, and C:N molar ratio of muscle tissue were also analyzed at the end of the experiment. Results showed that 1) the most responsive tissues were the muscle in the shrimp species and the visceral mass in the hermit crab species; 2) biomarker responses in both species occurred mostly after 7 days of exposure; 3) temperature stress led to an increase in biomarker levels; 4) highest biomarker fold-changes were detected in protective chaperones and antioxidants superoxide dismutase and glutathione-S-transferase; 4) integrated biomarker indices suggested poorer health status in individuals subjected to the heat wave; 5) performance changes at the organism level were only detected inR. durbanensis; and 6) mortality rates of both species remained unchanged with the heat wave. Finally, we concluded that these species are capable of physiological adjustments in response to rapid environmental changes, which ultimately confers them with enough thermal tolerance to withstand this simulated heat wave without major consequences for fitness.