First complete genome sequence of two Staphylococcus epidermidis bacteriophages
First complete genome sequence of two Staphylococcus epidermidis bacteriophages
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DOI：
10.1128/jb.01637-06
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发表时间：
2007-03-01
影响因子：
3.2
通讯作者：
Fischetti, Vincent A.
中科院分区：
文献类型：
--
作者：
Daniel, Anu;Bonnen, Penelope E.;Fischetti, Vincent A.
表皮葡萄球菌是引起医院感染的重要机会致病菌，通常与植入假体装置的患者的感染有关。在沙门氏菌中已鉴定出许多毒力决定因子。表皮，其通常通过水平基因转移获得。由于高重组潜力，噬菌体在这些转移事件中发挥重要作用。噬菌体基因组序列的知识提供了深入了解噬菌体宿主生物学和进化。我们目前的完整基因组序列和分子特征的两个S。epidermidis epidermidis，phi PH15（PH15）和phi CNPH82（CNPH82）。这两种病毒都属于Siphoviridae家族，并产生稳定的溶原菌。PH15和CNPH82基因组显示出高度的序列同源性，然而，我们的分析也揭示了重要的功能差异。PH15基因组包含两个内含子，并且证明了两个内含子的噬菌体mRNA的体内剪接。这两个内含子的二级结构也进行了预测，并表现出高度的相似性，嗜热链球菌噬菌体2972内含子。另一个发现是两种噬菌体之间的差异性重复感染抑制，这与溶原模块内的核苷酸序列和总体基因含量的差异相对应。我们对所有已知的Siphoviridae进行了系统发育分析，结果显示PH15和CNPH 82与金黄色葡萄球菌聚类，在S.金黄色葡萄球菌组，并提供了更高的整体分辨率的噬菌体蛋白质组树的噬菌体分支比以前的研究。到目前为止，没有S。epidermidis噬菌体基因组序列已在文献中报道，因此本研究代表了两个S.表皮细胞
Staphylococcus epidermidis is an important opportunistic pathogen causing nosocomial infections and is often associated with infections in patients with implanted prosthetic devices. A number of virulence determinants have been identified in S. epidermidis, which are typically acquired through horizontal gene transfer. Due to the high recombination potential, bacteriophages play an important role in these transfer events. Knowledge of phage genome sequences provides insights into phage-host biology and evolution. We present the complete genome sequence and a molecular characterization of two S. epidermidis phages, phi PH15 (PH15) and phi CNPH82 (CNPH82). Both phages belonged to the Siphoviridae family and produced stable lysogens. The PH15 and CNPH82 genomes displayed high sequence homology; however, our analyses also revealed important functional differences. The PH15 genome contained two introns, and in vivo splicing of phage mRNAs was demonstrated for both introns. Secondary structures for both introns were also predicted and showed high similarity to those of Streptococcus thermophilus phage 2972 introns. An additional finding was differential superinfection inhibition between the two phages that corresponded with differences in nucleotide sequence and overall gene content within the lysogeny module. We conducted phylogenetic analyses on all known Siphoviridae, which showed PH15 and CNPH82 clustering with Staphylococcus aureus, creating a novel clade within the S. aureus group and providing a higher overall resolution of the siphophage branch of the phage proteomic tree than previous studies. Until now, no S. epidermidis phage genome sequences have been reported in the literature, and thus this study represents the first complete genomic and molecular description of two S. epidermidis phages.