THE LOWEST CONDUCTANCE STATE OF THE ALAMETHICIN PORE
THE LOWEST CONDUCTANCE STATE OF THE ALAMETHICIN PORE
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DOI：
10.1016/0005-2736(80)90134-0
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
BOHEIM, G
中科院分区：
文献类型：
--
作者：
HANKE, W;BOHEIM, G
丙甲霉素孔的高分辨率实验证明了在低电导值下存在进一步的孔状态。在室温下，在1 M KCl中，发现该最低分辨电导状态为19 pS。该值与下一个更高的电导状态的值相差14-15倍，并且大约为。比短杆菌肽A的孔电导低20%。最低的电导状态似乎对Ca 2+、Cl−、Tris-H+和Hepes−是不可渗透的，而较高的电导状态则不是。
High resolution experiments of the alamethicin pore demonstrate the existence of a further pore state at low conductance values. This lowest resolved conductance state is found at 19 pS in 1 M KCl at room temperature. The value differs from that of the next higher conductance state by a factor of 14–15 and is approx. 20% lower than the gramicidin A pore conductance. The lowest conductance state seems to be impermeable to Ca2+, Cl−, Tris-H+and Hepes−, whereas the higher conductance states are not.