Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR
Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR
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DOI：
10.1128/jcm.37.8.2719-2722.1999
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发表时间：
1999-08-01
影响因子：
9.4
通讯作者：
Whittam, TS
中科院分区：
文献类型：
--
作者：
Reid, SD;Betting, DJ;Whittam, TS
设计多重PCR检测eae基因，同时鉴定致病性大肠杆菌的特异性等位基因。该方法在87株腹泻性大肠杆菌克隆中进行了试验。结果表明，PCR检测准确地检测到eae，并解析编码α、β和γ内膜变异的等位基因。
A multiplex PCR was designed to detect the eae gene and simultaneously identify specific alleles in pathogenic Escherichia coli. The method was tested on 87 strains representing the diarrheagenie E. coli clones. The results show that the PCR assay accurately detects eae and resolves alleles encoding the alpha, beta, and gamma intimin variants.