Oral Administration of Lactobacillus delbrueckii during the Suckling Phase Improves Antioxidant Activities and Immune Responses after the Weaning Event in a Piglet Model
Oral Administration of Lactobacillus delbrueckii during the Suckling Phase Improves Antioxidant Activities and Immune Responses after the Weaning Event in a Piglet Model
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在哺乳期口服德氏乳杆菌可改善仔猪模型断奶后的抗氧化活性和免疫反应
DOI：
10.1155/2019/6919803
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发表时间：
2019-01-01
影响因子：
--
通讯作者：
Huang, Xingguo
中科院分区：
文献类型：
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作者：
Li, Yinghui;Hou, Shuling;Huang, Xingguo
益生菌在肠道中的早期定植影响未来抗氧化剂和免疫系统功能的逐步发展和成熟。本研究以仔猪为模型，研究了哺乳期口服德氏乳杆菌（LAB）对宿主抗氧化和免疫应答的影响。100头新生仔猪在1、3、7和14日龄分别接受1、2、3和4 mL生理盐水（CON）或LAB。仔猪21日龄断奶，饲养至49日龄。血清，肝脏和肠道样本，分别在21日龄，28日龄和49日龄。结果表明，乳酸菌在第21、28和49天有降低血清8-羟基-2-脱氧鸟苷浓度和降低血清和肝脏丙二醛浓度的趋势，但增加了肝脏谷胱甘肽过氧化物酶的活性。与对照组相比，实验组仔猪肠黏膜中分泌型免疫球蛋白A和一些炎性细胞因子及趋化因子的浓度在21、28和49日龄时均显著升高（P < 0.05）。在整个试验期间，环氧化酶2和诱导型一氧化氮合酶的蛋白表达水平均显著升高（P < 0.05）。这些结果表明，乳酸菌的哺乳仔猪的管理，可以提高抗氧化能力和刺激肠道免疫反应，这些持久的效果也观察到断奶后长达4周。合理利用新生儿乳酸菌，对人类和动物未来的健康都是有益的。
Early colonization in the gut by probiotics influences the progressive development and maturity of antioxidant and immune system functionality in the future. This study investigated the impact of orally administrated Lactobacillus delbrueckii (LAB) during the suckling phase on future antioxidant and immune responses of the host, using a piglet model. One hundred neonatal piglets received saline (CON) or LAB at the amounts of 1, 2, 3, and 4 mL at 1, 3, 7, and 14 d of age, respectively. The piglets were weaned at the age of 21 d and fed until the age of 49 d. Serum, liver, and intestinal samples were obtained at 21, 28, and 49 d of age. The results showed that LAB tended to decrease serum 8-hydroxy-2-deoxyguanosine concentration and decreased the concentration of serum and hepatic malondialdehyde, but increased the activity of hepatic glutathione peroxidase on days 21, 28, and 49. The concentrations of secretory immunoglobulin A and some inflammatory cytokines and chemokines were increased (P < 0.05) in the intestinal mucosa of LAB-treated piglets on days 21, 28, and 49 compared to that of CON piglets. Likewise, protein expression of cyclooxygenase 2 and inducible nitric oxide synthase in the intestine of LAB-treated piglets was increased (P < 0.05) during the whole period. These results indicate that administration of LAB to the suckling piglet could improve antioxidant capacity and stimulate intestinal immune response, and these long-lasting effects are also observed up to 4 weeks after weaning. A proper utilization of LAB to neonates would be beneficial to human and animal's future health.