Single intramammary infusion of recombinant bovine interleukin-8 at dry-off induces the prolonged secretion of leukocyte elastase, inflammatory lactoferrin-derived peptides, and interleukin-8 in dairy cows.
Single intramammary infusion of recombinant bovine interleukin-8 at dry-off induces the prolonged secretion of leukocyte elastase, inflammatory lactoferrin-derived peptides, and interleukin-8 in dairy cows.
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DOI：
10.1155/2012/172072
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发表时间：
2012
影响因子：
3.1
通讯作者：
Kimura K
中科院分区：
文献类型：
--
作者：
Watanabe A;Hirota J;Shimizu S;Inumaru S;Kimura K
在干乳期，以50 μg/乳区/头（而非10 μg/乳区/头）单次乳房内输注重组牛白细胞介素-8（IL-8）诱导4头奶牛中的3头发生临床型乳房炎，导致直肠温度升高、乳腺发红和肿胀、广泛的多形白细胞（PMNL）浸润以及输注后1 - 28天（PI）的乳凝块形成。在乳腺的乳腺分泌物中，IL-8的高水平从感染后8小时持续到感染后28天，在感染后1-3天达到峰值。白细胞衍生的弹性蛋白酶和炎性22和23 kDa乳铁蛋白衍生肽（LDP）的水平也增加了乳腺分泌物中的乳腺炎。除了实验诱导的乳腺炎，乳腺分泌物从牛的腺体自发性金黄色葡萄球菌干期乳腺炎显示乳凝块形成和显着增加的PMNL计数，弹性蛋白酶，LDP，和IL-8的水平，与未感染的腺体的乳腺分泌物相比。这些结果表明，在乳房内输注IL-8引起炎症反应后，它诱导弹性蛋白酶、炎性LDP和IL-8的长期分泌，并且持久的IL-8诱导的炎症反应参与了S.金黄色干乳期乳腺炎。
A single intramammary infusion of recombinant bovine interleukin-8 (IL-8) at 50 μg/quarter/head, but not 10 μg/quarter/head, induced clinical mastitis in three of four cows during the dry-off period, resulting in an elevated rectal temperature, redness and swelling of the mammary gland, extensive polymorphonuclear leukocyte (PMNL) infiltration, and milk clot formation from 1 to 28 days post infusion (PI). In the mammary secretions of the mastitic glands, high levels of IL-8 were sustained from 8 hours to 28 days PI, peaking at 1–3 days PI. The levels of leukocyte-derived elastase and inflammatory 22 and 23 kDa lactoferrin derived peptides (LDP) were also increased in the mammary secretions from the mastitic glands. In addition to the experimentally induced mastitis, the mammary secretions from the glands of cattle with spontaneous Staphylococcus aureus dry-period mastitis displayed milk clot formations and significant increases in their levels of PMNL counts, elastase, LDP, and IL-8, compared with those of the mammary secretions from the uninfected glands. These results suggest that after an intramammary infusion of IL-8 has elicited inflammatory responses, it induces the prolonged secretion of elastase, inflammatory LDP, and IL-8, and that long-lasting IL-8-induced inflammatory reactions are involved in the pathogenesis of S. aureus dry-period mastitis.