Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma.
Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma.
复制标题
DOI：
10.2147/opth.s19453
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Chalam KV
中科院分区：
文献类型：
--
作者：
Balaiya S;Edwards J;Tillis T;Khetpal V;Chalam KV
肿瘤坏死因子 α (TNF-α) 是一种巨噬细胞/单核细胞衍生的多能细胞因子，与组织缺血、神经元损伤和重塑相关。正常眼和青光眼眼房水中 TNF-α 的生理水平尚不清楚。在这项研究中，我们评估了原发性开角型青光眼 (POAG) 患者房水中的 TNF-α 水平，并将其与对照组进行比较。从 32 名接受白内障摘除术、小梁切除术或房水分流术植入的 POAG 患者的眼睛中获取 50–100 μL 未稀释的房水样本。对照组取自 32 名接受常规白内障手术的正常受试者。使用单重珠免疫分析对 TNF-α 水平进行定量。 POAG 样品中的平均 TNF-α 水平为 2.72 ± 1.5 pg/mL（平均值 ± SD）。正常样本中的平均 TNF-α 水平为 1.59 ± 0.46 pg/mL（平均值 ± SD）。与正常样本相比，POAG 样本中的 TNF-α 水平显着增加 (P < 0.001)。根据高灵敏度 Luminex® 珠子免疫测定，与正常受试者相比，POAG 患者房水中 TNF-α 水平升高，并且可能是青光眼进展的可靠生物标志物。
Tumor necrosis factor alpha (TNF-α), a macrophage/monocyte derived pluripotent cytokine is associated with tissue ischemia, neuronal damage and remodeling. The physiological level of TNF-α in aqueous humor of normal and glaucomatous eyes is unknown. In this study, we evaluated the TNF-α levels in aqueous in patients with primary open angle glaucoma (POAG) and compared them to controls. 50–100 μL of undiluted aqueous humor samples were obtained from eyes of 32 POAG patients who underwent cataract extraction, trabeculectomy or aqueous shunt implantation. Controls were obtained from 32 normal subjects who underwent routine cataract surgery. TNF-α levels were quantified using singleplex bead immunoassay analysis. The average TNF-α level in POAG samples was 2.72 ± 1.5 pg/mL (mean ± SD). The average TNF-α level in normal samples was 1.59 ± 0.46 pg/mL (mean ± SD). Significant increase of TNF-α levels in POAG samples was noted in comparison to normal samples (P < 0.001). TNF-α levels are elevated in aqueous in patients with POAG compared to normal subjects based on highly sensitive Luminex® bead immunoassay and may be a reliable biomarker in the progression of glaucoma.