Quantum-Optically Enhanced STORM (QUEST) for Multi-Emitter Localization

Quantum-Optically Enhanced STORM (QUEST) for Multi-Emitter Localization
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用于多发射器定位的量子光学增强型 STORM (QUEST)

DOI:
10.1038/s41598-018-26271-1
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发表时间:
2018
期刊:
影响因子:
4.6
通讯作者:
Marc Aßmann
Marc Aßmann
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Marc Aßmann

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超分辨率成像引入了通过光学手段在纳米尺度上研究过程的新能力。然而,大多数超分辨率技术需要稀疏激发的几个发射器或高阶累积量的分析,以确定在附近的几个发射器。在这里,我们提出了一种方法,利用量子光学的方法对密集的发射体进行定位超分辨率成像,并自动确定它们的数量:量子光学增强风暴(QUEST)。通过利用归一化光子相关性,我们预测即使对于密度高达125个发射体/μm的密集发射体,在光子发射速率为105个光子/秒和发射体的情况下,定位精度也低于30 nm或更好。我们的技术不需要复杂的实验安排,只依赖于空间分辨的时间流的光子和随后的数据分析。
Super-resolution imaging has introduced new capabilities to investigate processes at the nanometer scale by optical means. However, most super-resolution techniques require either sparse excitation of few emitters or analysis of high-order cumulants in order to identify several emitters in close vicinity. Here, we present an approach that draws upon methods from quantum optics to perform localization super-resolution imaging of densely packed emitters and determine their number automatically: Quantum-optically enhanced STORM (QUEST). By exploiting normalized photon correlations, we predict a localization precision below 30 nm or better even for closely spaced emitter up to a density of 125 emitters perμm at photon emission rates of 105photons per second and emitter. Our technique does not require complex experimental arrangements and relies solely on spatially resolved time streams of photons and subsequent data analysis.
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