Depth-resolved fluorescence spectroscopy reveals layered structure of tissue
Depth-resolved fluorescence spectroscopy reveals layered structure of tissue
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DOI：
10.1364/opex.12.003218
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发表时间：
2004-07-12
期刊：
影响因子：
3.8
通讯作者：
Yu, MY
中科院分区：
文献类型：
--
作者：
Wu, YC;Xi, P;Yu, MY
利用共焦荧光光谱系统研究了生物组织中的深度分辨自发荧光。该系统提供了光学切片的能力，最大检测深度可达120 μ m，在检查的组织样本。发现最上面的角质化上皮层产生类似于胶原的强荧光。来自角化层和间质之间的上皮组织的荧光信号可以很好地分辨。研究结果表明，深度分辨荧光光谱技术具有为组织病理诊断提供更准确信息的潜力。(C)2004年美国光学学会。
A confocal fluorescence spectroscopy system is instrumented to study depth-resolved autofluorescence in biological tissue. The system provides the capability of optical sectioning with the maximal detection depth up to 120 mum in the examined tissue samples. It was found that the topmost keratinizing epithelial layer produces strong fluorescence similar to collagen. The fluorescence signal from epithelial tissue between the keratinizing layer and stroma can be well resolved. The study results show that depth-resolved fluorescence spectroscopy has the potential to provide more accurate information for the diagnosis of tissue pathology. (C) 2004 Optical Society of America.