Fabrication of "Clickable" Hydrogels via Dendron-Polymer Conjugates
Fabrication of "Clickable" Hydrogels via Dendron-Polymer Conjugates
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DOI：
10.1021/ma100292w
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发表时间：
2010-04-27
期刊：
影响因子：
5.5
通讯作者：
Sanyal, Rana
中科院分区：
文献类型：
--
作者：
Altin, Huseyin;Kosif, Irem;Sanyal, Rana
功能化水凝胶作为细胞生长和组织工程的三维（3D）支架有很大的兴趣。在水凝胶基质内精确控制生物相关分子密度的共价固定能力是非常需要的。通过Huisgen型“点击”反应制备的树突聚合物偶联物为反应性水凝胶提供了一种独特的前体。将第二代和第三代炔烃附加聚酯枝状聚合物与线性聚乙二醇二叠氮化合物PEG2K和PEG6K偶联，制备了一类枝状聚合物偶联物。烷基功能化树突聚合物树突与亲水性二叠氮偶联的控制交联提供了凝胶的凝胶效率大于80%。多余的剩余炔可以用来功能化这些水凝胶。链霉亲和素的固定化证明了交联和功能化程度的微调。
Functionalizable hydrogels are of great interest as three-dimensional (3D) scaffolds for cell growth and tissue engineering. The ability to covalently immobilize biologically relevant molecules with accurate control of their density within the hydrogel matrix is highly desirable. Dendron polymer conjugates prepared via Huisgen type "click" reaction provides a unique precursor for reactive hydrogels. A family of dendron polymer conjugates were prepared by coupling second- and third-generation alkyne appended polyester dendrons with linear poly(ethylenc glycol) diazides, PEG2K and PEG6K. Controlled cross-linking of alkyne-functionalized dendron polymer dendron conjugates with a hydrophilic diazide provides hydrogels with gelation efficiencies greater than 80%. Excess leftover alkynes can be used to functionalize these hydrogels as desired. Fine tuning of degree of cross-linking and functionalization is demonstrated by immobilization of streptavidin.