In Vitro Survival of Mesenchymal Stem Cells is Enhanced in Artificial Endolymph with Moderately High Concentrations of Potassium
In Vitro Survival of Mesenchymal Stem Cells is Enhanced in Artificial Endolymph with Moderately High Concentrations of Potassium
复制标题
中等高浓度钾在人工内淋巴中增强人间充质干细胞的体外存活
DOI：
10.1089/scd.2018.0016
复制
发表时间：
2018-05-07
影响因子：
4
通讯作者：
Zhao, Robert Chunhua
中科院分区：
文献类型：
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作者：
Gao, Juanjuan;Wang, Shihua;Zhao, Robert Chunhua
虽然间充质干细胞（MSC）是内耳毛细胞再生的有希望的候选者，但迄今为止，还没有令人信服的报告表明MSC是否可以在耳蜗中存活超过几周，因为内淋巴（EL）中高水平的钾（K+）被认为对移植的干细胞有毒。为了调节用于MSC移植的EL，我们进行了这项体外研究，以检查具有改变的K+浓度水平的人工EL（在5-153.8 mM的范围内）对源自各种人体组织的MSC的增殖、凋亡和形态学变化的影响。我们的研究结果表明，改变人工EL中的K+浓度可以显着影响MSC在体外的存活。我们发现，人工EL中55-130 mM的K+浓度可以提高MSC在体外的存活。然而，无论K+浓度如何，MSC都表现出增殖减少。
While mesenchymal stem cells (MSCs) are promising candidates for inner ear hair cell regeneration, to date, there have been no convincing reports indicating whether MSCs can survive in the cochlea for more than a few weeks, as the high levels of potassium (K+) in the endolymph (EL) are thought to be toxic to transplanted stem cells. For conditioning the EL for MSC transplantation, we conducted this in vitro study to examine the effects of artificial EL with altered K+ concentration levels, in the range of 5-153.8 mM, on proliferation, apoptosis, and morphological changes in MSCs derived from various human tissues. Our findings demonstrate that altering the K+ concentration in artificial EL could significantly influence the survival of MSCs in vitro. We discovered that K+ concentrations of 55-130 mM in artificial EL could enhance the survival of MSCs in vitro. However, MSCs exhibited reduced proliferation regardless of K+ concentration.