Complete genome sequence and cell structure of Limnochorda pilosa, a Gram-negative spore former within the phylum Firmicutes
Complete genome sequence and cell structure of Limnochorda pilosa, a Gram-negative spore former within the phylum Firmicutes
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Limnochorda pilosa（厚壁菌门内的革兰氏阴性孢子形成体）的完整基因组序列和细胞结构
DOI：
10.1099/ijsem.0.000881
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发表时间：
2016
影响因子：
2.8
通讯作者：
Hisaya Kojima and Manabu Fukui
中科院分区：
文献类型：
--
作者：
Miho Watanabe;Hisaya Kojima and Manabu Fukui
毛沼索菌（Limnochordapilosais）是一种兼性兼性多形厌氧菌，是目前暂归厚壁菌门（Firmicutes）的唯一种。本研究对L. pilosaHC 45 T的提取和分析。基因组大小为3.82Mbp，DNA G+C含量为69.73%。 基于30 S-50 S核糖体蛋白和23 S rRNA基因的系统发育分析一致表明L. pilosais在遗传学上与厚壁菌门的其他成员分离。超微结构观察表明，L.菌毛具有革兰氏阴性细胞壁和形成内生孢子的能力。因此，L。菌毛基因组具有革兰氏阴性菌特有的特征，并含有许多参与孢子形成的基因。另一方面，L.尽管它们被认为是厚壁菌门成员的内孢子形成系统所必需的。L. pilosa具有内含肽序列。基因组具有高百分比的GTG起始密码子，并且缺乏与细胞分裂相关的几个保守基因。
Limnochorda pilosais a pleomorphic facultative anaerobe and the sole species in the classLimnochordia, which has tentatively been placed in the phylumFirmicutes. In the present study, the complete genome sequence ofL. pilosaHC45Twas obtained and analysed. The genome size was 3.82 Mbp and the DNA G+C content was 69.73 %. Phylogenetic analyses based on the 30S-50S ribosomal proteins and 23S rRNA gene consistently indicated thatL. pilosais phylogenetically isolated from the other members of the phylumFirmicutes. Ultrastructural observation revealed thatL. pilosapossesses a Gram-negative-type cell wall and the capacity to form endospores. Accordingly, theL. pilosagenome has characteristics that are specific to Gram-negative bacteria and contains many genes that are involved in sporulation. On the other hand, several sporulation genes were absent from theL. pilosagenome although they have been regarded as essential for the endospore-forming system of members of the phylumFirmicutes. ThegyrB gene ofL. pilosapossesses an intein sequence. The genome has a high percentage of GTG start codons and lacks several conserved genes related to cell division.