Loss of the genetic diversity in cultivated populations of Pseudosciaena crocea by AFLP.
Loss of the genetic diversity in cultivated populations of Pseudosciaena crocea by AFLP.
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发表时间：
2009
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通讯作者：
Li Zhongbao;Fang Xiu;C. Jin;Chang Jianbo;Lei GuangGao;Zhang Guiling;Zhao Bin-li;Wang Zhanlin
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作者：
Li Zhongbao;Fang Xiu;C. Jin;Chang Jianbo;Lei GuangGao;Zhang Guiling;Zhao Bin-li;Wang Zhanlin
采用AFLP方法对福建省两个野生和两个栽培居群的遗传多样性进行了分析。在4个群体的120个个体中共检测到472个位点，其中多态性220个（46.61%）。AFLP分析结果表明，栽培群体的遗传多样性明显低于野生群体。野生群体的多态位点百分率、Nei’s基因多样性、Shannon’s信息指数和每个位点的平均有效等位基因数分别为42.42% ~ 45.14%、0.1046 ~ 0.1245、0.1659 ~ 0.1942和1.4153 ~ 1.4428，栽培群体的多态位点百分率和有效等位基因数分别为40.83% ~ 40.53%、0.1027 ~ 0.1039、0.1615 ~ 0.1633和1.3919 ~ 1.3856。4个居群间遗传距离为0.0359 ~ 0.1465，平均为0.079，基因流（Nm）为1.0808。这些结果表明，与其他鱼类相比，其遗传多样性水平较低。
Genetic diversity of two wild and two cultivated populations of Pseudosciaena crocea in Fujian Province were analyzed by AFLP. A total of 472 loci were detected from 120 individuals in the 4 populations, of which 220 (46.61%) are polymorphic. The results from AFLP technique show that the genetic diversity in the cultivated populations has been reduced compared to that of wild populations. The percentage of polymorphic loci, the Nei’s Gene Diversity, Shannon’s Information Index and the mean effective number of alleles per locus in two wild populations are 42.42%—45.14%, 0.1046—0.1245, 0.1659—0.1942 and 1.4153—1.4428, respectively, which in two cultivated populations are 40.83%—40.53%, 0.1027—0.1039, 0.1615—0.1633 and 1.3919—1.3856, respectively. Genetic distance among the 4 populations varies from 0.0359—0.1465, in the mean of 0.079, and the gene flow (Nm) is 1.0808. These results show a lower-level genetic diversity compared to other fishes.