Periodontal regeneration by application of recombinant human bone morphogenetic protein-2 to horizontal circumferential defects created by experimental periodontitis in beagle dogs
Periodontal regeneration by application of recombinant human bone morphogenetic protein-2 to horizontal circumferential defects created by experimental periodontitis in beagle dogs
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DOI：
10.1902/jop.1997.68.2.103
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发表时间：
1997-02-01
影响因子：
4.3
通讯作者：
Ishikawa, I
中科院分区：
文献类型：
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作者：
Kinoshita, A;Oda, S;Ishikawa, I
本研究的目的是检查应用重组人骨形态发生蛋白-2（rhBMP-2）后牙周组织的再生情况。在6只成年比格犬的12颗下颌第二前磨牙周围放置丝线结扎，直到骨丢失超过牙根长度的一半，进行实验性牙周破坏。然后进行皮瓣手术，去除暴露的牙骨质。骨嵴和牙骨质-釉质连接处（CEJ）之间的距离约为5 mm。将具有由明胶和聚乳酸聚乙醇酸共聚物制成的海绵型载体材料的RhBMP-2（40 μ g/100 μ l）置于分叉区域（5 mm × 5 mm × 5 mm）和牙根周围（10 mm × 5 mm × 2.5 mm × 2片）。在对照组中，以相同的方式放置不含rhBMP-2的相同载体材料。将皮瓣替换并缝合以完全覆盖这些材料。手术后12周，处死动物，并在颊舌平面上制备连续切片。在rhBMP-2处理部位观察到相当多的新骨形成。在器械处理的根面上观察到具有Sharpey纤维的新牙骨质。在组织计量学分析中，rhBMP-2治疗组的新骨、新牙骨质和结缔组织附着量显著更大（配对t检验; P < 0.01）。这些结果表明，适当应用rhBMP-2可以产生相当的牙周组织再生，即使在水平周向缺损的情况下。
THE PURPOSE OF THIS STUDY was to examine the regeneration of periodontal tissue after the application of recombinant human bone morphogenetic protein-2 (rhBMP-2) to horizontal circumferential defects created by experimental periodontitis. Twelve mandibular second premolars in 6 adult beagle dogs were subjected to experimental periodontal breakdown by placing silk ligatures around the teeth until the bone loss exceeded half of the root length. Flap surgery was then performed and the exposed cementum removed. The distance between the bone crest and cemento-enamel junction (CEJ) was about 5 mm. RhBMP-2 (40 mu g/100 mu l) With a sponge-type carrier material made of gelatin and polylactic acid polyglycolic acid copolymer was placed in the furcation area (5 mm x 5 mm x 5 mm) and around the roots (10 mm x 5 mm x 2.5 mm x 2 pieces). In the control group, the same carrier material without rhBMP-2 was placed in the same manner. The flaps were replaced and sutured to cover these materials completely. Twelve weeks after surgery, the animals were sacrificed and serial sections were prepared in a bucco-lingual plane. Considerable new bone formation was observed in the rhBMP-2-treated sites. New cementum with Sharpey's fibers was observed on the instrumented root surface. On histometric analysis, the amount of new bone, new cementum, and connective tissue attachment was significantly greater in the rhBMP-2-treated group (paired t test; P < 0.01). These results indicate that suitable application of rhBMP-2 can produce considerable periodontal tissue regeneration, even in cases of horizontal circumferential defects.