Cartilage tissue regeneration from bone marrow cells by RWV bioreactor using collagen sponge scaffold
Cartilage tissue regeneration from bone marrow cells by RWV bioreactor using collagen sponge scaffold
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DOI：
10.1016/j.msec.2008.09.029
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发表时间：
2009-05-05
期刊：
影响因子：
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通讯作者：
Uemura, Toshimasa
中科院分区：
文献类型：
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作者：
Ohyabu, Yoshimi;Tanaka, Junzo;Uemura, Toshimasa
三维培养技术在组织工程领域受到广泛关注。其中，RWV（旋转壁血管）生物反应器，模拟细胞的微重力环境，被认为是几种组织再生细胞的有用工具，如软骨细胞、成骨细胞等。在本文中，我们报道了利用胶原海绵支架，用RWV生物反应器成功地控制了兔骨髓细胞软骨组织的形状。此外，使用的支架被证明提高了糖胺聚糖（GAG）含量，增强了产品的抗压强度，仅一周的RWV培养即可达到0.9 MPa。胶原海绵支架的使用缩短了组织移植所需的培养时间。(C) 2008 Elsevier B.V.版权所有
Three dimensional culture techniques have attracted much attention in the field of tissue engineering. Among them, the RWV (rotating wall vessel) bioreactor, which simulates a microgravity environment for cells, is becoming recognized as a useful tool for several kinds of the cells for tissue regeneration such as chondrocytes, osteoblasts etc. In this paper, we report successful control of the cartilage tissue shape from rabbit bone marrow cells by RWV bioreactor using a collagen sponge scaffold. Moreover, the used scaffold was proved to enhance the glycosaminoglycan (GAG) content and strengthened the compression strength of the product, which reached 0.9 MPa in only one-week RWV culture. The usage of a collagen sponge scaffold shortened the necessary culture period for tissue transplantation. (C) 2008 Elsevier B.V. All rights reserved.