Monoclonal antibodies recognizing transforming growth factor-beta. Bioactivity neutralization and transforming growth factor beta 2 affinity purification.
Monoclonal antibodies recognizing transforming growth factor-beta. Bioactivity neutralization and transforming growth factor beta 2 affinity purification.
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DOI：
10.4049/jimmunol.142.5.1536
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发表时间：
1989-03
影响因子：
4.4
通讯作者：
J. Dasch;D. R. Pace;W. Waegell;D. Inenaga;L. Ellingsworth
中科院分区：
文献类型：
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作者：
J. Dasch;D. R. Pace;W. Waegell;D. Inenaga;L. Ellingsworth
获得了4株能识别转化生长因子-β2的单抗。其中一株单抗1D11.16在体外能中和转化生长因子β1和β2的生物学活性。这在依赖IL-1，PHA的胸腺细胞有丝分裂试验中得到了证实，该试验可被转化生长因子-β以剂量依赖的方式抑制。在Western blotting中，四种单抗均可识别转化生长因子-β2的二聚体。单抗还能免疫沉淀[125I]-转化生长因子-β2。将单抗3C7.14偶联到琼脂糖凝胶上，可以有效地从复杂的蛋白质混合物中免疫亲和纯化转化生长因子-β2。测定了4株单抗的亲和常数，范围为3.4×10(8)~1.6×10(7)L/摩尔。
Four mAb able to recognize transforming growth factor-beta 2 (TGF-beta)2 were obtained. One of these mAb, 1D11.16, was able to neutralize the biological activity of both TGF-beta 1 and beta 2 in vitro. This was demonstrated in an Il-1, PHA-dependent thymocyte mitogenic assay that is inhibitable by TGF-beta in a dose-dependent manner. All four mAb recognized the dimeric form of TGF-beta 2 in Western blots. The mAb were also found to immunoprecipitate [125I]-TGF-beta 2. mAb 3C7.14 coupled to Sepharose could efficiently immunoaffinity purify TGF-beta 2 from a complex mixture of proteins. Affinity constants were determined for the four mAb and they ranged from 3.4 x 10(8) to 1.6 x 10(7) L/mol.