Immuno-proteomics analysis between OMV of vaccine and dominant wild type strains of Bordetella pertussis in Iran
Immuno-proteomics analysis between OMV of vaccine and dominant wild type strains of Bordetella pertussis in Iran
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DOI：
10.18502/ijm.v12i2.2610
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发表时间：
2020-04-01
影响因子：
1.4
通讯作者：
Siadat, Seyed Davar
中科院分区：
文献类型：
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作者：
Badamchi, Ali;Bahrami, Fariborz;Siadat, Seyed Davar
背景和目标：尽管针对百日咳的疫苗接种计划广泛，但近年来该疾病在世界范围内死灰复燃。我们的目的是研究百日咳博德特氏菌（Bp）的外膜囊泡（OMV）的蛋白质组成，并评估OMV抗原的免疫原性，无论是在疫苗和优势的野生型菌株在Iran.Materials和方法：OMV纯化疫苗和野生型菌株。通过暴露患者和接种疫苗婴儿的血清，研究OMV的免疫反应性。利用双向电泳技术比较了OMV的蛋白质谱。结果：两种模式菌株在二维凝胶蛋白图谱上存在差异。使用LC-MS/MS对来自优势伊朗菌株的所选蛋白质的进一步分析表明，所鉴定的蛋白质属于不同的功能类别，包括（i）代谢，（ii）膜转运和分泌系统，（iii）生物合成和降解，（iv）适应、粘附、致病性、保守假设和保护反应。此外，还鉴定了许多免疫原性蛋白，包括Bp 2434（丝氨酸蛋白酶）和Bp 1616结果表明，OMV疫苗株和野生型株的DNA结合蛋白（推定的DNA结合蛋白）均为OMV疫苗的潜在抗原OMV Bp可被视为百日咳的替代疫苗，含有细菌的蛋白抗原，与全细菌细胞疫苗相比，可以提供同等的功效，并且比无细胞百日咳疫苗具有副作用更少、成本更低等优点。
Background and Objectives: Despite widespread vaccination programs against pertussis, there has been a worldwide resurgence of the disease in recent years. We aimed to investigate protein composition of outer membrane vesicles (OMV) of Bordetella pertussis (Bp) and to evaluate the immunogenicity of OMV antigens both in the vaccine and the dominant wild type strains in Iran.Materials and Methods: The OMV were purified from both vaccine and wild type strains. The immunoreactivity of the OMVs was investigated by exposing sera taken from the patients and the vaccinated infants. The protein profiles of OMVs were compared using two-dimensional electrophoresis. The LC-MS/MS was used to analyse and identify differentially expressed protein spots.Results: The two type strains showed differences in their 2D gel protein profile. Further analysis of selected proteins from the dominant Iranian strains using LC-MS/MS demonstrated that the identified proteins fell into different functional categories including (i) metabolism, (ii) membrane transport and secretion system, (iii) biosynthesis and degradation, (iv) adaption, adhesion, pathogenicity, conserved hypothetical and protection responses. Moreover, a number of immunogenic proteins were identified including Bp 2434 (serine protease) and Bp 1616 (putative DNA binding protein) from the vaccine and the wild type strains, respectively which could be considered as potential antigens for an OMV vaccine.Conclusion: OMV Bp could be considered as an alternative vaccine against pertussis, containing the bacterium's protein antigens that can confer equal efficacy compared to a whole bacterial cell vaccine with advantages such as less side effects and lower costs than acellular pertussis vaccines.