Yeast contains two functional genes coding for ribosomal protein S10.
Yeast contains two functional genes coding for ribosomal protein S10.
复制标题
酵母含有两个编码核糖体蛋白S10的功能基因。
作者：
R. Leer;M. M. van Raamsdonk;C. Molenaar;H. Witsenboer;W. Mager;R. Planta
确定了编码酵母核糖体蛋白S10的基因的第二个拷贝的DNA序列，并将其与第一个基因拷贝的序列进行比较。此外，还鉴定了这些基因转录开始和终止的位点。两个基因拷贝的氨基酸编码区实际上是相同的。然而，前导序列和特别是尾随序列显着不同，而中间序列几乎没有任何同源性。利用序列差异，我们可以确定这两个基因都在营养生长的酵母细胞中表达。然而，各自的转录本的相对数量有所不同。
The DNA sequence of the second copy of the gene coding for yeast ribosomal protein S10 was determined and compared with the sequence of the first gene-copy. In addition, the sites at which the transcription of these genes start and terminate are identified. The amino acid coding regions of the two gene copies are virtually identical. The leader and in particular the trailer sequences, however, are significantly different, while the intervening sequences have hardly any homology. Taking advantage of the sequence differences we could establish that both genes are expressed in the vegetatively growing yeast cell; the respective transcripts, however, differ in their relative amounts.