Splenic, thymic, bony and lymph node metastases from orthotopic human lung carcinomas in immunocompromised mice.
Splenic, thymic, bony and lymph node metastases from orthotopic human lung carcinomas in immunocompromised mice.
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发表时间：
2000
影响因子：
2
通讯作者：
R. Hastings;D. Burton;D. Summers-Torres;R. Quintana;E. Biederman;L. Deftos
中科院分区：
文献类型：
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作者：
R. Hastings;D. Burton;D. Summers-Torres;R. Quintana;E. Biederman;L. Deftos
在人肺癌的原位动物模型中，几周后就频繁观察到骨和淋巴结转移，但向其他器官的转移很少见。这项研究评估了免疫功能低下小鼠原位肺癌模型在六个月内远处转移的发展情况。人A549肺腺癌细胞被稳定转染，表达高水平的绿色荧光蛋白。将 1 x 10(6) 细胞的悬浮液注入无胸腺和 SCID 小鼠的肺部，以产生原位人肺癌。六个月后实验结束时，所有动物均患有原发性肿瘤。 70% 的动物存在脾转移和淋巴结转移，三只 SCID 小鼠中的两只有胸腺转移。三只动物有骨转移。因此，高比例的患有原位肺癌的免疫功能低下的小鼠最终会发生转移。
In orthotopic animal models of human lung cancer, bone and lymph node metastases have been observed with high frequency after periods of a few weeks, but metastases to other organs are rare. This study evaluated development of distant metastases over a six-month period in a model of orthotopic lung carcinoma in immunocompromised mice. Human A549 lung adenocarcinoma cells were stably transfected to express high levels of green fluorescent protein. Suspensions of 1 x 10(6) cells were instilled into the lungs of athymic and SCID mice to produce orthotopic human lung carcinomas. All animals had primary tumors at termination of the experiment six months later. Splenic metastases and lymph node metastases were present in 70% of the animals and two of the three SCID mice had thymic metastases. Three animals had bony metastases. Thus, a high percentage of immunocompromised mice with orthotopic lung carcinomas ultimately develop metastases.