Viral gene expression during acute simian varicella virus infection
Viral gene expression during acute simian varicella virus infection
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DOI：
10.1099/0022-1317-83-4-841
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发表时间：
2002-04-01
影响因子：
3.8
通讯作者：
Soike, KF
中科院分区：
文献类型：
--
作者：
Gray, WL;Mullis, L;Soike, KF
猿猴水痘病毒（SVV）在非人灵长类动物中引起一种天然水痘样疾病。猴水痘的爆发在灵长类动物设施中零星发生。以猴水痘为模型研究水痘-带状疱疹病毒（VZV）的发病机制和潜伏期。在这项研究中，SVV基因表达和组织病理学分析急性感染的黑长尾猴的组织。RT-PCR分析表明，在急性感染猴的皮肤，肺，肝和神经节组织中表达特异性SVV立即早期，早期和晚期基因。通过免疫组织化学分析，在感染猴的皮肤、肺、肝和脾中检测病毒抗原表达和组织病理学，包括坏死和炎症。病毒抗原表达，但很少或没有组织病理学，是明显的神经节，病毒潜伏期的最终网站。本研究为进一步研究病毒基因在水痘发病机制和潜伏期中的作用奠定了基础。
Simian varicella virus (SVV) causes a natural varicella-like disease in nonhuman primates. Outbreaks of simian varicella occur sporadically in primate facilities. Simian varicella is used as a model for investigation of varicella-zoster virus (VZV) pathogenesis and latency. In this study, SVV gene expression and histopathology were analysed in tissues of acutely infected vervet monkeys. RT-PCR analysis demonstrated expression of specific SVV immediate early, early and late genes in the skin, lung, liver and ganglia tissues of acutely infected monkeys. Viral antigen expression and histopathology, including necrosis and inflammation, were detected in the skin, lungs, liver and spleen of infected monkeys by immunohistochemical analysis. Viral antigen expression, but little or no histopathology, was evident in the neural ganglia, the eventual site of viral latency. The study provides a foundation for further investigation on the role of viral genes in varicella pathogenesis and latency.