Expression of bone morphogenetic proteins-2/4 in neural stem cells and their lineages
Expression of bone morphogenetic proteins-2/4 in neural stem cells and their lineages
复制标题
骨形态发生蛋白-2/4在神经干细胞及其谱系中的表达
DOI：
10.55782/ane-2009-1755
复制
发表时间：
2009-01-01
影响因子：
1.4
通讯作者：
Hu, Jian-Guo
中科院分区：
文献类型：
--
作者：
Lue, He-Zuo;Hu, Jian-Guo
神经干细胞(NSCs)是一种多能祖细胞，具有自我更新和生成不同类型神经细胞的能力。骨形态发生蛋白(BMPs)在决定神经干细胞的命运中起着至关重要的作用。虽然一些报道描述了BMP在早期发育的中枢神经系统(CNS)中的表达，但它们主要涉及其在限制性区域的表达。然而，有关BMP在神经干细胞及其谱系中表达的信息很少。在本研究中，基于神经干细胞能够自我更新和分化为星形胶质细胞、神经元或少突胶质细胞的体外模型，我们模拟了神经干细胞在体内的发育环境。利用一种可以识别BMP-2和BMP-4的抗体，我们发现BMP-2/4只在星形胶质细胞中表达。炎性细胞因子(肿瘤坏死因子-α和/或干扰素-γ)不影响BMPs在神经干细胞及其谱系中的表达模式。提示星形胶质细胞可能是神经干细胞分化过程中和中枢神经系统损伤后炎症环境中BMPs的来源之一。
Neural stem cells (NSCs) are multipotent progenitor cells that possess the ability to self-renew and generate different neural cell types. Bone morphogenetic proteins (BMPs) play critical roles in the determination of the fate of NSCs. Although some reports delineate BMP expression in the early developing central nervous system (CNS), they deal mainly with its expressions in restricted areas. However, little information is available for BMP expression in NSCs and their lineages. In this study, based on an in vitro model in which NSCs can self-renew and differentiate into astrocytes, neurons or oligodendrocytes, we mimicked the environment of NSC development in vivo. Using an antibody which can recognize BMP-2 and BMP-4, we showed that BMP-2/4 is only expressed in astrocytes. Inflammatory cytokines (tumor necrosis factor-alpha and/or interferon-gamma) do not influence the expression pattern of BMPs in NSCs and their lineages. These results suggest that astrocytes might be One Source of BMPs during the differentiation of NSCs and in the inflammatory environment after CNS injury.