Ultrastructural Changes in Axons Following Exposure to Pulsed Radiofrequency Fields
Ultrastructural Changes in Axons Following Exposure to Pulsed Radiofrequency Fields
复制标题
DOI：
10.1111/j.1533-2500.2009.00317.x
复制
发表时间：
2009-11-01
期刊：
影响因子：
2.6
通讯作者：
Cosman, Eric R., Jr.
中科院分区：
文献类型：
--
作者：
Erdine, Serdar;Bilir, Ayhan;Cosman, Eric R., Jr.
脉冲射频(PRF)通过电极施加于神经结构，如外周感觉痛感受器轴突和背根神经节，在临床上对减轻疼痛和其他神经性综合征是有效的。然而，对发生这种情况的基本机制的充分了解尚未得到澄清。本研究将PRF应用于大鼠坐骨神经的传入轴突。使用标准射频电极和射频发生器将射频信号输出施加到坐骨神经，使用已成功应用于临床的标准PRF参数。10天后用电子显微镜观察处理后轴突的超微结构。同时对对侧坐骨神经施加对照、假敷贴，以提供统计学差异比较。结果发现，PRF暴露后轴突内部超微结构成分出现微观损伤，包括：线粒体膜和形态异常，微丝和微管断裂和解体。C-纤维的损伤似乎比A-Delta和A-β纤维更明显。从内部电场强度和热力学参数的角度对结果进行了讨论。
Pulsed radiofrequency (PRF) fields applied by an electrode to neural structures, such as the peripheral sensory nociceptor axons and dorsal root ganglion, are clinically effective in reducing pain and other neuropathic syndromes. However, a full understanding of the underlying mechanisms by which this occurs has not yet been clarified. In this study, PRF is applied to the afferent axons of the sciatic nerves of rats. A standard radiofrequency (RF) electrode and RF generator is used to apply the RF signal output to the sciatic nerve using standard PRF parameters that have been successfully used in clinical practice. The ultrastructure of the treated axons is observed after 10 days by electron microscopy. A control, sham application is simultaneously applied to the contralateral sciatic nerve to provide a statistical differential comparison. It is found that the internal ultrastructural components of the axons show microscopic damage after PRF exposure, including: abnormal membranes and morphology of mitochondria, and disruption and disorganization of microfilaments and microtubules. The damage appears to be more pronounced for C-fibers than for A-delta and A-beta fibers. The results are discussed in terms of internal electric field strengths and thermodynamic parameters.