Polar protein transport between apical and basal cells during tobacco early embryogenesis
Polar protein transport between apical and basal cells during tobacco early embryogenesis
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烟草早期胚胎发生过程中顶端细胞和基底细胞之间的极性蛋白转运
DOI：
10.1007/s00299-012-1362-5
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
孙蒙祥
中科院分区：
文献类型：
--
作者：
孙蒙祥
KeymessageWe发现顶基细胞之间的蛋白质运输可以是单向的，这揭示了在早期胚胎发生过程中，两个细胞在它们之间的细胞间通讯中扮演着不同的角色。关于这些细胞类型之间与其细胞命运决定有关的可能通信，已经有很多推测。在这里，我们报告使用光活化绿色荧光蛋白（PA-GFP）在烟草的顶端和基底细胞之间的细胞间通讯在早期胚胎发生的痕迹。我们发现PA-GFP在两细胞原胚的顶端和基底细胞之间运输，并且蛋白质运输是单向的朝向顶端细胞，突出了不同的细胞通讯作用。进一步的超微结构分析显示，在连接顶细胞和基底细胞的壁中存在大量的胞间连丝，这可能为蛋白质运输提供了通道。我们的数据显示了一种可能的独特的方法，在早期胚胎发生的顶端和基底细胞之间的细胞与细胞的通信。
Key messageWe found that protein trafficking between apical and basal cell can be unidirectional, which reveals the different roles of the two cells in the cell-to-cell communication between them during early embryogenesis.AbstractIn most angiosperm species, asymmetric zygote division results in an apical cell and a basal cell that have distinct cell fates. Much has been speculated about possible communication between these cell types in relation to their cell fate determination. Here, we report on the use of photoactivatable green fluorescent protein (PA-GFP) in tobacco to trace intercellular communication between apical and basal cells during early embryogenesis. We found that PA-GFP was transported between apical and basal cells of a two-celled proembryo, and that protein trafficking was unidirectional toward the apical cell, highlighting different cell communication roles. Further ultrastructural analysis showed numerous plasmodesmata in the walls connecting the apical and basal cells, which may provide channels for protein trafficking. Our data show a possible unique method of cell-to-cell communication between apical and basal cells during early embryogenesis.
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影响因子：
2.9
作者：
Liarzi, O;Epel, BL
通讯作者：
Epel, BL
DOI：
10.1073/pnas.0505622102
发表时间：
2005-08-16
影响因子：
11.1
作者：
Kim, I;Kobayashi, K;Zambryski, PC
通讯作者：
Zambryski, PC
影响因子：
6.6
作者：
Steffen Lau;J. Ehrismann;A. Schlereth;Shinobu Takada;U. Mayer;G. Jürgens
通讯作者：
Steffen Lau;J. Ehrismann;A. Schlereth;Shinobu Takada;U. Mayer;G. Jürgens
影响因子：
11.4
作者：
Fu Chun-mei;Sun Meng-xiang;Z. Chang;Yang Hong-yuan
通讯作者：
Fu Chun-mei;Sun Meng-xiang;Z. Chang;Yang Hong-yuan
影响因子：
7.2
作者：
He, Yu-Chi;He, Yu-Qing;Yang, Hong-Yuan
通讯作者：
Yang, Hong-Yuan