Clostridium phytofermentans sp nov., a cellulolytic mesophile from forest soil
Clostridium phytofermentans sp nov., a cellulolytic mesophile from forest soil
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DOI：
10.1099/00207713-52-4-1155
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发表时间：
2002-07-01
影响因子：
2.8
通讯作者：
Leschine, SB
中科院分区：
文献类型：
--
作者：
Warnick, TA;Methé, BA;Leschine, SB
从森林土壤中分离到一株专性厌氧、中温、降解纤维素的细菌ISDg(T)。该菌株的细胞革兰氏染色阴性，尽管具有革兰氏阳性细胞壁的超微结构，并且是可移动的直杆，形成球形的末端孢子，使孢子囊膨胀。纤维素、果胶、多聚半乳糖醛酸、淀粉、木聚糖、阿拉伯糖、纤维二糖、果糖、半乳糖、龙胆二糖、葡萄糖、乳糖、麦芽糖、甘露糖、核糖和木糖支持生长。发酵的最终产物主要是乙醇、乙酸盐、CO2和H-2，甲酸盐和乳酸是次要产物。最适生长温度为35-37℃。基于16S rRNA序列比较的系统发育分析表明，ISDg(T)菌株与一组厌氧菌相关联，包括草食梭菌、多糖梭菌和大众梭菌。该菌株的G+C含量为35.9mol%。根据菌株ISDg(T)与其近缘菌株ISDg(T)在基因和表型上的众多差异，提出了菌株ISDg(T)作为梭状芽孢杆菌属的一个新种，命名为植物发酵梭菌属(Clostridium pltofermentans sp.)11月是提议的。模式菌株为ISDg(T)(=ATCC 700394(T))。
An obligately anaerobic, mesophilic, cellulolytic bacterium, strain ISDg(T), was isolated from forest soil. Cells of this isolate stained Gram-negative, despite possessing a Gram-positive cell-wall ultrastructure, and were motile, straight rods that formed spherical terminal spores that swelled the sporangium. Cellulose, pectin, polygalacturonic acid, starch, xylan, arabinose, cellobiose, fructose, galactose, gentiobiose, glucose, lactose, maltose, mannose, ribose and xylose supported growth. The major end products of fermentation were ethanol, acetate, CO2 and H-2; formate and lactate were minor products. The optimum temperature for growth was 35-37 degreesC. Phylogenetic analyses based on 16S rRNA sequence comparisons showed that strain ISDg(T) was related to a group of anaerobes that included Clostridium herbivorans, Clostridium polysaccharolyticum and Clostridium populeti. The G+C content of this strain was 35.9 mol%. On the basis of numerous genotypic and phenotypic differences between strain ISDg(T) and its close relatives, strain ISDg(T) is proposed as a novel species in the genus Clostridium, for which the name Clostridium phytofermentans sp. nov. is proposed. The type strain is ISDg(T) (= ATCC 700394(T)).