Human neutrophil elastase: degradation of basement membrane components and immunolocalization in the tissue.
Human neutrophil elastase: degradation of basement membrane components and immunolocalization in the tissue.
复制标题
人中性粒细胞弹性蛋白酶：基底膜成分的降解和组织中的免疫定位。
DOI：
10.1093/oxfordjournals.jbchem.a123277
复制
发表时间：
1990
影响因子：
2.7
通讯作者：
Yutaka Nagai
中科院分区：
文献类型：
--
作者：
Hideto Watanabe;Shunji Hattori;S. Katsuda;Isao Nakanishi;Yutaka Nagai
人中性粒细胞弹性蛋白酶被纯化为两种同工酶，分别命名为E1和E2。同工酶对IV型胶原、层粘连蛋白、纤维连接蛋白和硫酸乙酰肝素蛋白多糖的降解作用相似。弹性酶对这种基底膜成分的降解可能有助于炎症过程中中性粒细胞的外渗。在被测试的底物中，只有对中性粒细胞明胶酶敏感的V型胶原对弹性酶具有抵抗力。这种酶的广泛底物专一性也可能导致炎症部位的组织破坏。我们制备了针对纯化酶的单抗，并将其应用于免疫组织化学研究。在支气管炎和结节性多动脉炎中，弹性蛋白酶与断裂的弹性纤维有关，这表明该酶在体内确实破坏了组织。在类风湿关节渗出液中，弹性蛋白酶染色呈弥漫性细小颗粒。用该单抗进行免疫组织化学研究，将为揭示与中性粒细胞浸润相关疾病的发病机制提供补充。
Human neutrophil elastase was purified to homogeneity as two isozymes named E1 and E2. The isozymes degraded Type IV collagen, laminin, fibronectin, and heparan sulfate proteoglycan similarly to each other. The degradation of such basement membrane components by elastase may assist the extravasation of neutrophils in the process of inflammation. Among the substrates tested, only type V collagen, which is susceptible to neutrophil gelatinase, was resistant to elastase. This broad substrate specificity of the enzyme may also contribute to tissue destruction at the sites of inflammation. We produced a monoclonal antibody against the purified enzyme and applied it to immunohistochemical studies. In bronchopneumonia and polyarteritis nodosa, elastase was associated with the cleaved elastic fibers, indicating that the enzyme really destroys tissue in vivo. In the exudates of rheumatoid joint, elastase was stained as diffuse fine granules. Immunohistochemical studies with the monoclonal antibody will provide a complementary way to disclose the mechanism of diseases related to neutrophil infiltration.