Tissue response and biomaterial integration: the efficacy of in vitro methods
Tissue response and biomaterial integration: the efficacy of in vitro methods
复制标题
DOI：
10.1016/s1389-0344(02)00019-9
复制
发表时间：
2002-08-01
期刊：
影响因子：
--
通讯作者：
Peters, K
中科院分区：
文献类型：
--
作者：
Kirkpatrick, CJ;Krump-Konvalinkova, V;Peters, K
植入涉及组织创伤，引起炎症反应，伴随着伤口愈合反应，包括血管生成、成纤维细胞激活和基质重塑。到目前为止，这种反应的类型和程度，以提供各种生物材料的最佳整合实际上是未知的。三个主要的研究领域可以提供有用的数据来更好地理解这些现象：外植生物材料的研究，动物模型和相关的体外技术。本文将介绍后一个领域的例子和内皮细胞（EC）培养系统的应用，以研究重要的组织（如促炎细胞因子，趋化因子）和材料（如金属离子，颗粒碎片）因子对炎症和血管生成反应的调节作用。中心特征是使用微血管内皮细胞（MEC），可用于二维和三维（3-D）分析。我们还利用基因操作从人肺中培养了永久MEC细胞系（HPMEC-ST1），正在测试其是否适合研究细胞-生物材料的相互作用。此外，为了研究给药系统（DDS），正在开发合适的体外技术。特别感兴趣的是中枢神经系统的目标，我们的方法是建立一个血脑屏障（BBB）的人体模型。我们科学理念的一个支柱是，这种体外方法可以为理解组织-生物材料界面的生物反应做出重要贡献，从而进一步为组织工程（TE）和药物输送应用提供因果方法。(C) 2002 Elsevier Science B.V.版权所有
Implantation involves tissue trauma, which evokes an inflammatory response, coupled to a wound healing reaction, involving angiogenesis, fibroblast activation and matrix remodelling. Until now the type and extent of such reactions to give optimal integration of various biomaterials are practically unknown. Three principal fields of research can yield useful data to understand these phenomena better: studies on explanted biomaterials, animal models and relevant in vitro techniques. This paper will present examples of the latter field and the application of endothelial cell (EC) culture systems to study the effects of important tissue (e.g. pro-inflammatory cytokines, chemokines) and material (e.g. metal ions, particulate debris) factors on the regulation of the inflammatory and angiogenic response. A central feature is the use of microvascular endothelial cells (MEC), which can be used in both 2-and 3-dimensional (3-D) assays. We have also used genetic manipulation to develop a permanent MEC line from the human lung (HPMEC-ST1), which is being tested for its suitability to study cell-biomaterial interactions. In addition, suitable in vitro techniques are being developed in order to investigate drug delivery systems (DDS). Of particular interest is the targeting of the central nervous system, our approach being to establish a human model of the blood-brain barrier (BBB). A mainstay of our scientific philosophy is that such in vitro methods can make an important contribution to understanding biological reactions at the tissue-biomaterial interface and thus further a causal approach to tissue engineering (TE) and drug delivery applications. (C) 2002 Elsevier Science B.V. All rights reserved.