Application of M13 phage display for identifying immunogenic proteins from tick (Ixodes scapularis) saliva
Application of M13 phage display for identifying immunogenic proteins from tick (Ixodes scapularis) saliva
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DOI：
10.1186/s12896-015-0167-3
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发表时间：
2015-05-30
影响因子：
3.5
通讯作者：
Hust, Michael
中科院分区：
文献类型：
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作者：
Becker, Martin;Felsberger, Andre;Hust, Michael
背景资料：蜱作为大量不同病原体的载体，可能最值得注意的是莱姆病的病原体伯氏疏螺旋体。在美国，最主要的蜱虫媒介是黑腿蜱，肩突硬蜱。蜱叮咬是一个特殊的公共卫生问题，因为没有针对大多数蜱传播病原体的疫苗。基于某些非天然宿主动物如豚鼠或人类可以对蜱叮咬产生适应性免疫反应的观察，抗蜱疫苗接种是一种潜在的方法来解决与蜱叮咬相关的健康风险。肩胛肌的蛋白质被人类免疫血清识别。从饲喂18 h若虫I的唾液腺mRNA中产生寡肽文库。肩胛肌使用人免疫血清选择八种免疫原性寡肽。三个选定的免疫原性寡肽克隆和生产的重组蛋白。用人血清验证了鉴定的金属蛋白酶（MP1）的免疫原性。该酶先前已被描述，并被假设为免疫原性，这在本研究中得到了证实。有趣的是，它在其他硬蜱中也有相似的同源物。通过寡肽噬菌体展示鉴定肩胛肌。MP1是疫苗开发的潜在候选者。
Background: Ticks act as vectors for a large number of different pathogens, perhaps most notably Borrelia burgdorferi, the causative agent of Lyme disease. The most prominent tick vector in the United States is the blacklegged tick, Ixodes scapularis. Tick bites are of special public health concern since there are no vaccines available against most tick-transmitted pathogens. Based on the observation that certain non-natural host animals such as guinea pigs or humans can develop adaptive immune responses to tick bites, anti-tick vaccination is a potential approach to tackle health risks associated with tick bites.Results: The aim of this study was to use an oligopeptide phage display strategy to identify immunogenic salivary gland proteins from I. scapularis that are recognized by human immune sera. Oligopeptide libraries were generated from salivary gland mRNA of 18 h fed nymphal I. scapularis. Eight immunogenic oligopeptides were selected using human immune sera. Three selected immunogenic oligopeptides were cloned and produced as recombinant proteins. The immunogenic character of an identified metalloprotease (MP1) was validated with human sera. This enzyme has been described previously and was hypothesized as immunogenic which was confirmed in this study. Interestingly, it also has close homologs in other Ixodes species.Conclusion: An immunogenic protein of I. scapularis was identified by oligopeptide phage display. MP1 is a potential candidate for vaccine development.