A role of poly (ADP-ribose) polymerase in NF-κB transcriptional activation
A role of poly (ADP-ribose) polymerase in NF-κB transcriptional activation
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DOI：
10.1515/bc.1999.118
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发表时间：
1999-07-01
影响因子：
3.7
通讯作者：
Hottiger, MO
中科院分区：
文献类型：
--
作者：
Hassa, PO;Hottiger, MO
转录因子核因子-kappaB在免疫和炎症反应中起关键作用。在这里，我们证明了聚腺苷二磷酸核糖聚合酶(PARP)是体内特异的核因子-kappaB转录激活所必需的。在PARP(-/-)细胞中，HIV-LTR启动子和依赖于NF-kappa B的人工启动子的活性显著降低，而不依赖于诱导NF-kappa B的信号通路。此外，PARP在PARP(-/-)细胞中的表达可在体内恢复依赖于NF-kappa B的基因激活。最后，我们证明了核因子-kappaB和PARP都形成了稳定的可免疫沉淀的核复合体。这种相互作用不需要DNA结合。我们的结果表明，PARP是依赖于NF-kappa B的靶基因激活级联中的一个重要的辅助因子。
The transcription factor NF-kappa B plays a critical role in immune and inflammatory responses. Here we show that poly (ADP ribose) polymerase (PARP) is required for specific NF-kappa B transcriptional activation in vivo. The activation of the HIV-LTR promoter and an NF-kappa B-dependent artificial promoter was drastically reduced in PARP (-/-) cells, independently of the signaling pathway through which NF-kappa B was induced. Furthermore NF-kappa B-dependent gene activation was restored in vivo by the expression of PARP in PARP (-/-) cells. Finally, we show that both NF-kappa B and PARP formed a stable immunoprecipitable nuclear complex. This interaction did not need DNA binding. Our results suggest that PARP is an important cofactor in the activation cascade of NF-kappa B-dependent target genes.