GENTAMICIN RESISTANCE IN CLINICAL-ISOLATES OF PSEUDOMONAS-AERUGINOSA ASSOCIATED WITH DIMINISHED GENTAMICIN ACCUMULATION AND NO DETECTABLE ENZYMATIC MODIFICATION
GENTAMICIN RESISTANCE IN CLINICAL-ISOLATES OF PSEUDOMONAS-AERUGINOSA ASSOCIATED WITH DIMINISHED GENTAMICIN ACCUMULATION AND NO DETECTABLE ENZYMATIC MODIFICATION
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DOI：
10.7164/antibiotics.29.743
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发表时间：
1976-01-01
影响因子：
3.3
通讯作者：
VANDENELZEN, HM
中科院分区：
文献类型：
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作者：
BRYAN, LE;HARAPHONGSE, R;VANDENELZEN, HM
对庆大霉素耐药的铜绿假单胞菌菌株 (3) 作为代表性庆大霉素耐药临床分离株从加拿大埃德蒙顿阿尔伯塔大学医院 (UAH) 获得，进行了表征以确定其耐药机制。所有菌株均表现出广泛的氨基糖苷类耐药性（妥布霉素、西索米星、阿米卡星、链霉素、卡那霉素、SCH 20569），但没有庆大霉素乙酰化、腺苷酸化或磷酸化活性的证据。庆大霉素与从抗性或敏感菌株获得的核糖体或可溶性细胞级分同样有效地抑制无细胞系统中的氨基酸掺入。在 2 个菌株中检测到质粒 DNA，但抗性不能通过接合转移至铜绿假单胞菌或大肠杆菌受体。与敏感菌株相比，抗性菌株表现出庆大霉素的能量依赖性积累显着减少。这些在 UAH 中常见的菌株很可能具有耐药性，因为直到外部庆大霉素浓度相对较高时，庆大霉素才能穿过细胞质膜转运至核糖体位点。
Strains (3) of P. aeruginosa resistant to gentamicin obtained as representative gentamicin-resistant clinical isolates from the University of Alberta Hospital (UAH) in Edmonton, Canada were characterized to determine their mechanism of resistance. All strains showed wide aminoglycoside resistance (tobramycin, sisomicin, amikacin, streptomycin, kanamycin, SCH 20569) but contained no evidence of gentamicin-acetylating, adenylylating or phosphorylating activity. Gentamicin inhibited amino-acid incorporation in cell-free systems equally well with either ribosomes or soluble cell fractions obtained from either resistant or sensitive strains. Plasmid DNA was detected in 2 strains, but resistance could not be transferred by conjugation to either P. aeruginosa or Escherichia coli recipients. The resistant strains showed a marked reduction in energy-dependent accumulation of gentamicin compared to a sensitive strain. These strains which are common at UAH are most likely resistant due to a failure of gentamicin to be transported across the cytoplasmic membrane to ribosomal sites until relatively high external gentamicin concentrations.