Screening and identification of differential metabolites in serum and urine of bamaxiang pigs bitten by trimeresurus stejnegeri based on UPLC-Q-TOF/MS metabolomics technology
Screening and identification of differential metabolites in serum and urine of bamaxiang pigs bitten by trimeresurus stejnegeri based on UPLC-Q-TOF/MS metabolomics technology
复制标题
基于UPLC-Q-TOF/MS代谢组学技术筛选鉴定被竹叶青咬伤的八马香猪血清和尿液中差异代谢物
DOI：
10.2131/jts.47.389
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发表时间：
2022-01-01
影响因子：
2
通讯作者：
Liao, Ming
中科院分区：
文献类型：
--
作者：
Guan, ZheZhe;Li, YaLan;Liao, Ming
竹叶青蛇是我国十大毒蛇之一，被其咬伤可引起急重症。阐明竹叶青蛇咬伤引起的机体代谢变化，将有利于竹叶青蛇咬伤的诊断和治疗。建立竹叶青蛇咬伤猪模型，采用超高效液相色谱-四极杆-飞行时间-质谱（UPLC-Q-TOF-MS）技术，对血清和尿液中代谢产物进行ESI+和ESI-模式的筛选和鉴定。血清中有油酸、石胆酸、脱氧胆酸、次黄嘌呤等9种代谢产物，尿中有多巴胺、硫代半胱氨酸、精氨酸、吲哚乙醛等11种代谢产物，血清富集途径分析表明色氨酸尿、囊性纤维化肝病、Hartnup病、高压氧暴露和胆汁性肝硬化等5种代谢途径。这些途径中的核心代谢物，包括脱氧胆酸、石胆酸、色氨酸和次黄嘌呤，发生了显著变化。尿液富集途径分析显示，有4条代谢途径，包括芳香族L-氨基酸脱羧酶、白癜风、蓝尿布综合征和高胆固醇血症，这些代谢途径中的核心代谢物包括多巴胺、5-羟基吲哚乙酸和精氨酸。综上所述，本研究成功建立了竹叶青蛇咬伤的动物模型，并确定了竹叶青蛇咬伤的代谢标志物和代谢途径。这些代谢产物和途径可能具有潜在的应用价值，为竹叶青蛇咬伤的治疗提供了一定的理论依据。
Trimeresurus stejnegeri is one of the top ten venomous snakes in China, and its bite causes acute and severe diseases. Elucidating the metabolic changes of the body caused by Trimeresurus stejnegeri bite will be beneficial to the diagnosis and treatment of snakebite. Thus, an animal pig model of Trimeresurus stejnegeri bite was established, and then the metabolites of serum and urine were subse-quently screened and identified in both ESI+ and ESI-modes identified by ultra-performance liquid chro-matography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS) methods. There are 9 dif-ferential metabolites in serum, including Oleic acid, Lithocholic acid, Deoxycholic acid, Hypoxanthine, etc. There are 11 differential metabolites in urine, including Dopamine, Thiocysteine, Arginine, Indoleac-etaldehyde, etc. Serum enrichment pathway analysis showed that 5 metabolic pathways, including Tryp-tophanuria, Liver disease due to cystic fibrosis, Hartnup disease, Hyperbaric oxygen exposure and Biliary cirrhosis, the core metabolites in these pathways, including deoxycholic acid, lithocholic acid, trypto-phan and hypoxanthine, changed significantly. Urine enrichment pathway analysis showed that 4 meta-bolic pathways, including Aromatic L-Amino Acid Decarboxylase, Vitiligo, Blue Diaper Syndrome and Hyperargininemia, the core metabolites in these pathways including dopamine, 5-hydroxyindole acetic acid and arginine. Taken together, the current study has successfully established an animal model of Tri-meresurus stejnegeri bite, and identified the metabolic markers and metabolic pathways of Trimeresurus stejnegeri bite. These metabolites and pathways may have potential application value and provide a thera-peutic basis for the treatment of Trimeresurus stejnegeri bite.